Food and Fermentation Industries >

2025 , Vol. 51 >Issue 21: 365 - 371

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042285

Analysis of ACE-inhibitory peptides in douchi based on NanoLC-Orbitrap-MS

  • RAN Haoyu ,
  • YANG Shen ,
  • WU Yu ,
  • ZHOU Xuhao ,
  • YANG Haozhi ,
  • YIN Liguo ,
  • ZHU Wenyou ,
  • LI Cen ,
  • YAN Yan
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  • 1(School of Liquor and Food Engineering, Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang 550025, China)
    2(Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin 644000, China)
    3(College of Life and Food Engineering, Guizhou University, Guiyang 550025, China)

Received date: 2025-02-03

  Revised date: 2025-03-27

  Online published: 2025-11-21

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Abstract

Douchi, a traditional Chinese fermented soybean product, is beloved by consumers for its unique flavor and possesses bioactivities such as antioxidant and antihypertensive effects.To further explore the functional compounds in Douchi, this study focused on three common types of Douchi in China—Aspergillus, Mucor, and bacterial.Ultrafiltration was used to isolate water-soluble peptides, and a total of 2 587 peptides were identified using nanoflow liquid chromatography-orbitrap mass spectrometry (NanoLC-Orbitrap-MS) in the three types of Douchi.Among these, 42 peptides were common across all three types.The angiotensin-converting enzyme (ACE) inhibitory activity was analyzed, revealing that the peptide VVPPGHPF exhibited the highest inhibition activity, with an IC50 value of (43.01±1.16) μmol/L.The ACE inhibition model confirmed that VVPPGHPF is a competitive inhibitor of ACE, capable of competing with key residues in the catalytic site of ACE.Molecular docking simulations indicated that VVPPGHPF forms hydrogen bonds with the S1 pocket of ACE (Glu384 and Ala354), demonstrating a binding affinity of -6.72 kcal/mol.The results of this study provide a theoretical basis for the preparation of bioactive peptides from Douchi and further analysis of their health benefits.

Key words： Douchi; nanoflow liquid chromatography-orbitrap mass spectrometry; polypeptide; angiotensin converting enzyme; molecular docking

Cite this article

RAN Haoyu , YANG Shen , WU Yu , ZHOU Xuhao , YANG Haozhi , YIN Liguo , ZHU Wenyou , LI Cen , YAN Yan . Analysis of ACE-inhibitory peptides in douchi based on NanoLC-Orbitrap-MS[J]. Food and Fermentation Industries, 2025 , 51(21) : 365 -371 . DOI: 10.13995/j.cnki.11-1802/ts.042285

References

[1] JIANG L W, CHEN Y, ZHAO T T, et al.Analysis of differential metabolites in Liuyang Douchi at different fermentation stages based on untargeted metabolomics approach[J].Food Chemistry:X, 2025, 25:102097.
[2] 谢艳华, 谢靓, 李跑, 等.GC-MS分析毛霉型、细菌型、曲霉型豆豉中脂肪酸组成[J].中国油脂, 2017, 42(7):115-119;123.
XIE Y H, XIE L, LI, P, et al.GC-MS analysis of fatty acid composition in mucor-type, bacterial-type and aspergillus -type douchi[J].China Oils and Fats, 2017, 42(7):115-119;123.
[3] CHATTERJEE C, GLEDDIE S, XIAO C W.Soybean bioactive peptides and their functional properties[J].Nutrients, 2018, 10(9):1211.
[4] LI T N, ZHANG X R, REN Y Y, et al.Antihypertensive effect of soybean bioactive peptides:A review[J].Current Opinion in Pharmacology, 2022, 62:74-81.
[5] 周伊恒, 吕垚, 杨梓钰, 等.英国和爱尔兰高血压学会《成人高血压转诊路径及治疗管理》共识声明解读[J].中国全科医学, 2025, 28(10):1173-1178.
ZHOU Y H, LYU Y, YANG X Y, et al.Interpretation of the consensus statement of the British and Irish hypertension societies on adult hypertension referral pathway and therapeutic management[J].Chinese General Practice, 2025, 28(10):1173-1178.
[6] 马丽媛, 王增武, 樊静, 等.《中国心血管健康与疾病报告2022》要点解读[J].中国全科医学, 2023, 26(32):3975-3994.
MA L Y, WANG Z W, FAN J, et al.Interpretation of report on cardiovascular health and diseases in China 2022[J].Chinese General Practice, 2023, 26(32):3975-3994.
[7] WEI G M, ZHAO F R, ZHANG Z Y, et al.Identification and characterization of umami-ACE inhibitory peptides from traditional fermented soybean curds[J].Food Chemistry, 2025, 465(Pt 2):142160.
[8] SANDHU R, MANN B, SHARMA R, et al.Identification and molecular docking of ACE inhibitory peptides derived from sodium substituted cheddar cheese[J].International Journal of Peptide Research and Therapeutics, 2024, 30(6):59.
[9] CHANAJON P, HAMZEH A, TIAN F, et al.Hypotensive effect of potent angiotensin-I-converting enzyme inhibitory peptides from corn gluten meal hydrolysate:Gastrointestinal digestion and transepithelial transportation modifications[J].Food Chemistry, 2025, 462:140953.
[10] HUANG X C, GAO T T, CHEN X, et al.Taste characteristics of salty peptides from Porphyra haitanensis and the synergistic saltiness enhancement with CaCl2[J].Food Chemistry, 2024, 461:140901.
[11] ZHANG Y Y, MA S J, LI H X, et al.Preparation, separation, and identification of antioxidant peptides from protein hydrolysate of Polygonatum cyrtonema Hua[J].Food Bioscience, 2024, 62:105351.
[12] MAO S Y, JIN W, FU S S, et al.Strategies for mapping protein hydrolysate profiles and pharmacokinetics based on non-targeted proteomics combining skyline-aided quantitative techniques[J].Analytica Chimica Acta, 2023, 1265:341272.
[13] DEKKER P M, BOEREN S, SACCENTI E, et al.Network analysis of the proteome and peptidome sheds light on human milk as a biological system[J].Scientific Reports, 2024, 14:7569.
[14] YANG X K, BAI H C, YIN L J, et al.Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry[J].Food Science and Human Wellness, 2023, 12(3):851-860.
[15] WEI D, FAN W L, XU Y.In vitro production and identification of angiotensin converting enzyme (ACE) inhibitory peptides derived from distilled spent grain prolamin isolate[J].Foods, 2019, 8(9):390.
[16] LAN X D, SUN L X, MUHAMMAD Y, et al.Studies on the interaction between angiotensin-converting enzyme (ACE) and ACE inhibitory peptide from Saurida elongata[J].Journal of Agricultural and Food Chemistry, 2018, 66(51):13414-13422.
[17] 张璐, 甘雨嫣, 钟玉旺, 等.青刺果ACE抑制肽的分离纯化、结构鉴定及其体外活性评价[J].中国食品学报, 2024, 24(7):321-331.
ZHANG L, GAN Y Y, ZHONG Y W, et al.Isolation, identification, structure characterization and in vitro activity evaluation of ACE Inhibitory peptides from Prinsepia utilis[J].Journal of Chinese Institute of Food Science and Technology, 2024, 24(7):321-331.
[18] 刘华宇, 廖彭莹, 张天丰, 等.鳖蛋黄血管紧张素转化酶抑制肽分离纯化及活性分析[J].食品科学, 2025, 46(1):40-48.
LIU H Y, LIAO P Y, ZHANG T F, et al.Separation, purification, and activity analysis of angiotensin converting enzyme inhibitory peptides in enzymatically hydrolyzed egg yolk of Chinese soft-shelled turtle[J].Food Science, 2025, 46(1):40-48.
[19] LI M Y, FAN W L, XU Y.Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste[J].LWT, 2021, 151:112221.
[20] XIANG L, QIU Z C, ZHAO R J, et al.Advancement and prospects of production, transport, functional activity and structure-activity relationship of food-derived angiotensin converting enzyme (ACE) inhibitory peptides[J].Critical Reviews in Food Science and Nutrition, 2023, 63(10):1437-1463.
[21] WANG X M, CHEN H X, FU X G, et al.A novel antioxidant and ACE inhibitory peptide from rice bran protein:Biochemical characterization and molecular docking study[J].LWT, 2017, 75:93-99.
[22] YU F M, ZHANG Z W, LUO L W, et al.Identification and molecular docking study of a novel angiotensin-I converting enzyme inhibitory peptide derived from enzymatic hydrolysates of Cyclina sinensis[J].Marine Drugs, 2018, 16(11):411.
[23] SHIH Y H, CHEN F A, WANG L F, et al.Discovery and study of novel antihypertensive peptides derived from Cassia obtusifolia seeds[J].Journal of Agricultural and Food Chemistry, 2019, 67(28):7810-7820.
[24] XUE L, WANG X D, HU Z H, et al.Identification and characterization of an angiotensin-converting enzyme inhibitory peptide derived from bovine casein[J].Peptides, 2018, 99:161-168.
[25] 张迎阳, 董亚云, 邹平, 等.橄榄仁抗氧化肽的分离鉴定及分子对接[J].食品与发酵工业, 2024, 50(5):189-198.
ZHANG Y Y, DONG Y Y, ZOU P, et al.Isolation, identification and molecular docking of antioxidant peptides from olive kernel[J].Food and Fermentation Industries, 2024, 50(5):189-198.
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