This study used a selective precipitation method to isolate κ-and β-casein with a ratio similar to human caseins from goat micellar casein concentrates (MCC), and then also tried scale-up production.Optimization of process parameters for critical steps including redissolution, casein-calcium complexation, αs-casein precipitation and low-temperature equilibration resulted in κ-and β-casein with high recovery and purity.At laboratory scale, the yields for κ- and β-caseins reached 85.7% and 97.3%, with purities being 18.0% and 68.7%, respectively.In scale-up production with 60 L MCC solution, κ- and β-caseins yields reached 76.4% and 88.7%, with purities of 18.0% and 67.6%, respectively.Washing the precipitate one or more times reduced ash content to be less than 5%.This method brings the proportion of goat caseins closer to that of human caseins, thereby enhancing the application value of MCC in fields such as infant.
MIAO Guangyong
,
TAO Xiumei
,
PENG Xiaoyu
,
GAO Yu
,
WANG Shanshan
,
WU Tong
,
LIU Dasong
,
ZHOU Peng
. Separation, preparation, and scale-up production of goat κ- and β-casein ingredients[J]. Food and Fermentation Industries, 2025
, 51(22)
: 79
-88
.
DOI: 10.13995/j.cnki.11-1802/ts.042471
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