Food and Fermentation Industries >

2025 , Vol. 51 >Issue 23: 78 - 87

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042166

Bifidobacterium animalis subsp. lactis IOB LO7 postbiotic enhances immune function in immunosuppressed mice

  • CAO Jin ,
  • ZHAO Zihao ,
  • XU Dexu ,
  • ZHAO Yuqi ,
  • XIE Linlin ,
  • HAN Xuemei ,
  • LUO Xuegang ,
  • HE Hongpeng ,
  • LIANG Wu ,
  • WANG Nan
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  • 1(College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Tianjin InnoOrigin Biological Biotechnology Co.Ltd., Tianjin 300301, China)

Received date: 2025-01-16

  Revised date: 2025-04-06

  Online published: 2025-12-25

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Abstract

Bifidobacterium animalis subsp. lactis IOB LO7 was made of a strain of probiotic bacteria isolated from breast milk.To investigate the effect of IOB LO7 postbiotics on immune function.In this study, RAW264.7 was used as a research subject, and the IOB LO7 postbiotic element was able to increase the secretion of nitric oxide (NO), IL-6, and TNF-α (P<0.01 or P<0.001) by RAW264.7 cells.Mouse splenic lymphocytes were taken as the study object, and IOB LO7 postbiotics were able to significantly promote lymphocyte proliferation (P<0.05 or P<0.001).A cyclophosphamide-induced immunocompromised mouse model was used as a further study subject and the normal control group (NC group), cyclophosphamide model group (CTX group), drug control group (CTX+LH), IOB LO7 postbiotic low dose group (CTX+BBL), and IOB LO7 postbiotic high dose group (CTX+BBH) were established.Results showed that IOB LO7 postbiotics were able to restore cyclophosphamide-induced body weight loss, increase the spleen index (P<0.05 or P<0.01), increase the ratio of CD4+ to CD8+ T cells in splenic lymph(P<0.05), restore structural compartments of the spleen, increase the levels of TLR2 (P<0.01), RELA, and NFKB1 (P<0.05 or P<0.001) mRNA in splenic tissues, and increase the levels of MUC1 (P<0.05), MUC2 (P<0.05 or P<0.01), and MUC3 (P<0.05) mRNA in colonic tissues, as well as significantly increase the number of leukocytes in blood (P<0.001 or P<0.01).The above results indicated that Bifidobacterium animalis subsp. lactis IOB LO7 postbiotic could activate macrophage M1 polarization and promote splenic lymphocyte proliferation, and also had an immune-enhancing effect on cyclophosphamide-induced immunosuppressed mice, and its mechanism of action was related to the restoration of T-lymphocyte subpopulations, activation of the NF-κB signalling pathway, and the improvement of the intestinal mucosal barrier.

Key words： probiotics; Bifidobacterium; postbiotics; immune activation; NF-κB signaling pathway

Cite this article

CAO Jin , ZHAO Zihao , XU Dexu , ZHAO Yuqi , XIE Linlin , HAN Xuemei , LUO Xuegang , HE Hongpeng , LIANG Wu , WANG Nan . Bifidobacterium animalis subsp. lactis IOB LO7 postbiotic enhances immune function in immunosuppressed mice[J]. Food and Fermentation Industries, 2025 , 51(23) : 78 -87 . DOI: 10.13995/j.cnki.11-1802/ts.042166

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