Food and Fermentation Industries >

2025 , Vol. 51 >Issue 23: 88 - 96

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042565

Exploring the effect of quinic acid on the intestinal flora of obese people based on in vitro fermentation

  • ZHANG Ze ,
  • LU Yao ,
  • ZHENG Wei ,
  • XU Lin ,
  • ZHANG Yue ,
  • LI Sen
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  • 1(School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai 200093, China)

Received date: 2025-02-28

  Revised date: 2025-04-07

  Online published: 2025-12-25

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Abstract

Obesity, as a global public health issue, is closely associated with gut microbiota dysbiosis.This study aimed to investigate the regulatory effects of millet polyphenol quinic acid (QA) on the gut microbiota of obese individuals.An in vitro fermentation model was established using fecal microbiota from obese donors with QA.The structural and functional changes of microbiota were analyzed by real-time monitoring of fermentation dynamics (pH and OD600), 16S rRNA sequencing, and HPLC-based metabolite quantification.Results showed that QA intervention significantly accelerated pH decline in the initial fermentation phase and promoted microbial growth in the later phase.Gut microbiota analysis revealed that QA reduced the Firmicutes/Bacteroidetes (F/B) ratio and significantly enriched Blautia and Weissella.Furthermore, QA enhanced the activity of quinate dehydrogenase and shikimate dehydrogenase, promoting shikimic acid production and modulating microbial metabolic pathways.These findings demonstrate that millet-derived QA ameliorates obesity-related metabolic disorders by reshaping gut microbiota composition and metabolic activity, providing a theoretical basis for developing gut-microbiota-targeted functional foods.

Key words： millet polyphenols; quinic acid; in vitro fermentation; gut microbiota; obesity

Cite this article

ZHANG Ze , LU Yao , ZHENG Wei , XU Lin , ZHANG Yue , LI Sen . Exploring the effect of quinic acid on the intestinal flora of obese people based on in vitro fermentation[J]. Food and Fermentation Industries, 2025 , 51(23) : 88 -96 . DOI: 10.13995/j.cnki.11-1802/ts.042565

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