Food and Fermentation Industries >

2025 , Vol. 51 >Issue 24: 86 - 94

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042369

Regulatory effect of piceatannol on lipid metabolism of HepG2 cells induced by sodium palmitate through PPARα

  • CHEN Fuchun ,
  • XU Ben ,
  • SHEN Weijun ,
  • LIU Lei ,
  • WAN Fachun ,
  • CHENG Anwei
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  • 1(College of Food Science and Technology, Hunan Agricultural University, Changsha 410000, China)
    2(College of Animal Science and Technology, Hunan Agricultural University, Changsha 410000, China)
    3(College of Veterinary Medicine, Hunan Agricultural University Changsha 410000, China)

Received date: 2025-02-14

  Revised date: 2025-05-07

  Online published: 2026-01-12

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Abstract

This study aimed to investigate the effects of taxifolin (TAX) on palmitic acid (PA)-induced lipid metabolism in HepG2 cells and its regulatory mechanisms.Using the CCK8 assay determined the appropriate modeling concentration of PA and the safe concentration of TAX.Oil Red O staining validated the establishment of the model.Triglyceride (TG) and reactive oxygen species (ROS) levels were measured to assess lipid accumulation and oxidative stress.Real-time quantitative reverse transcription PCR (RT-qPCR) analyzed the gene expression of acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD), CCAAT/enhancer-binding protein α (C/EBP-α), sterol regulatory element-binding protein 1C (SREBP-1C), and peroxisome proliferator-activated receptor α (PPARα).Western blot (WB) detected the protein expression of fatty acid synthase (FAS), cluster of differentiation 36 (CD36), adenosine monophosphate-activated protein kinase (AMPK), and PPARα.Immunofluorescence was used to determine the protein expression levels of FAS and PPARα.Small interfering RNA (siRNA) was used to silence PPARα to further validate the regulatory mechanism of TAX on lipid metabolism.TAX at 150 μmol/L significantly reduced TG levels in PA-treated HepG2 cells (P<0.01).All concentrations of TAX significantly decreased ROS levels (P<0.01).At 150 μmol/L, TAX significantly downregulated the mRNA expression of ACC, SCD, C/EBP-α, and SREBP-1C, while upregulating PPARα mRNA expression (P<0.01).All concentrations of TAX significantly upregulated PPARα protein expression and AMPK phosphorylation levels (P<0.05), while significantly downregulating FAS and CD36 protein expression (P<0.01).Immunofluorescence results showed that all concentrations of TAX significantly upregulated PPARα and downregulated FAS fluorescence intensity (P<0.01).siRNA-mediated PPARα silencing inhibited the protective effects of TAX.Taxifolin can mediate the PPARα signaling pathway to regulate lipid accumulation and ameliorate PA-induced lipid metabolism disorder in HepG2 cells.

Key words： taxifolin; lipid metabolism; signaling pathway; gene silencing; HepG2 Cell

Cite this article

CHEN Fuchun , XU Ben , SHEN Weijun , LIU Lei , WAN Fachun , CHENG Anwei . Regulatory effect of piceatannol on lipid metabolism of HepG2 cells induced by sodium palmitate through PPARα[J]. Food and Fermentation Industries, 2025 , 51(24) : 86 -94 . DOI: 10.13995/j.cnki.11-1802/ts.042369

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