Food and Fermentation Industries >

2025 , Vol. 51 >Issue 24: 322 - 330

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042655

Reparative effects and mechanisms of exopolysaccharides from Limosilactobacillus reuteri CCFM1453 on gastritis

  • ZHANG Shuyue ,
  • TANG Xin ,
  • MAO Bingyong ,
  • ZHANG Qiuxiang ,
  • CHEN Wei ,
  • ZHAO Jianxin ,
  • CUI Shumao
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  • (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2025-03-08

  Revised date: 2025-05-05

  Online published: 2026-01-12

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Abstract

This study investigates the reparative effects and underlying mechanisms of exopolysaccharides (EPS) produced by Limosilactobacillus reuteri CCFM1453 on aspirin-induced gastritis in mice.A comparative model of pseudo-germ-free and conventional mice was established to evaluate the role of EPS under gut microbiota intervention.The results demonstrated that EPS effectively alleviated gastric inflammation, reduced the spleen index, and mitigated gastric histopathological damage.Mechanistically, EPS regulated inflammatory responses by modulating the JAK/STAT, TLR/MyD88/NF-κB, and PI3K/AKT signaling pathways, leading to the regulation of pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10 expression, as well as the suppression of excessive cyclooxygenase-2 (COX-2) production.Consequently, the levels of prostaglandin E2 (PGE2) in pseudo-germ-free and conventional mice were reduced by 65.47% and 60.62%, respectively, compared to the model group, while the levels of trefoil factor 3 (TFF3) in pseudo-germ-free mice decreased by 42.13%, contributing to the amelioration of gastritis.Moreover, the findings from the pseudo-germ-free mouse model further confirmed that the anti-inflammatory and reparative effects of EPS were primarily mediated through direct activation of immune signaling pathways rather than gut microbiota-dependent mechanisms.

Key words： Limosilactobacillus reuteri CCFM1453; exopolysaccharide; aspirin-induced gastritis; anti-inflammatory mechanisms

Cite this article

ZHANG Shuyue , TANG Xin , MAO Bingyong , ZHANG Qiuxiang , CHEN Wei , ZHAO Jianxin , CUI Shumao . Reparative effects and mechanisms of exopolysaccharides from Limosilactobacillus reuteri CCFM1453 on gastritis[J]. Food and Fermentation Industries, 2025 , 51(24) : 322 -330 . DOI: 10.13995/j.cnki.11-1802/ts.042655

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