With the expansion of the drinking population, plant-based beverages with alcohol detoxification and hepatoprotective effects have broad market prospects.This study employed single-factor analysis and response surface methodology to optimize the processing conditions of fermented juice beverages.Additionally, a human alcohol breath test and a zebrafish drunkenness model were used to investigate their effects on alcohol detoxification and hepatoprotection, providing a theoretical basis for the application of such beverages.The results showed that the optimal processing conditions for the fermented juice beverage were:a solid-to-liquid ratio of 1∶30(g∶mL) for the fermented material, 0.09% Ampelopsis grossedentata powder, 0.03% turmeric, and 0.01% sucralose, yielding a sensory score of (88.75±0.31).Compared with the control group, within 30 minutes of consuming the fermented juice beverage, the rate of decline in exhaled alcohol concentration in humans accelerated, and sobering time was shortened by 2 h.Furthermore, within the maximum tolerated concentration, the beverage exhibited significant sobering effects on ethanol-induced excitement-phase drunken zebrafish, with a more pronounced effect observed at higher doses.Meanwhile, the auxiliary protective effect against alcoholic fatty liver increased with the concentration of beverage, indicating that fermented juice beverages at appropriate concentrations possess alcohol-detoxifying and liver-protective properties.
[1] WU X, YANG L J, GAO P, et al.Effect of Xingnaojing injection for the treatment of acute alcoholism:A protocol of systematic review and meta-analysis[J].Medicine, 2020, 99(26):e20785.
[2] CENI E, MELLO T, GALLI A.Pathogenesis of alcoholic liver disease:Role of oxidative metabolism[J].World Journal of Gastroenterology, 2014, 20(47):17756-17772.
[3] 倪以宇, 赵大庆, 倪伟锋, 等.葛根-枳椇子药对发酵工艺及解酒功效评价研究[J].食品与发酵工业, 2022, 48(16):209-215.
NI Y Y, ZHAO D Q, NI W F, et al.Fermentation technology of Pueraria Lobata-Hovenia dulcis and its anti-alcoholic effect[J].Food and Fermentation Industries, 2022, 48(16):209-215.
[4] 朱雪梅, 袁小茜, 于梦淇, 等.以葛根提取物和藤茶粉为主要材料的配方的醒酒功能研究[J].现代预防医学, 2023, 50(2):250-256.
ZHU X M, YUAN X X, YU M Q, et al.Study on sobering function of products with pueraria root extract and rattan tea powder as main materials[J].Modern Preventive Medicine, 2023, 50(2):250-256.
[5] CARNEIRO R C V, YE L Y, BAEK N, et al.Vine tea (Ampelopsis grossedentata):A review of chemical composition, functional properties, and potential food applications[J].Journal of Functional Foods, 2021, 76:104317.
[6] 许尨, 郑丽君, 马海然, 等.葛根姜黄复合物解酒护肝效果[J].食品工业, 2023, 44(3):164-168.
XU M, ZHENG L J, MA H R, et al.The effect of the compound of pueraria and turmeric to relieve hangover and protect liver[J].The Food Industry, 2023, 44(3):164-168.
[7] LIESCHKE G J, CURRIE P D.Animal models of human disease:Zebrafish swim into view[J].Nature Reviews.Genetics, 2007, 8(5):353-367.
[8] 钱星文,谭转换,朱晨,等.益甘舒抗乙醇诱导斑马鱼兴奋期醉酒的作用研究[J].今日药学,2020, 30(7):474-477.
QIAN X W, TAN Z H, ZHU C, et al.Effect of yiganshu on the anti-alcohol in zebrafish[J].Pharmacy Today, 2020, 30(7):474-477.
[9] 王雪松,张素敏,隋韶奕,等.黑果腺肋花楸酵素饮料的研制[J].农业科技与装备,2020(6):28-30.
WANG X S, ZHANG S M, SUI S Y, et al.Development of Aronia melanocarpa enzyme beverage[J].Agricultural Science & Technology and Equipment, 2020(6):28-30.
[10] 刘斐, 孙松.具有醒酒与解酒双重功效的天然饮料及其制备方法:中国, CN101822402A[P].2010-09-08
LIU F, SUN S.Natural beverage with dual anti-alcohol and sobering effects and preparation method thereof:China, CN101822402A[P].2010-09-08.
[11] 潘敏,谢少龙,丘慧秋,等.枳椇子、葛花的最佳配比解酒方影响人体乙醇吸收代谢过程研究[J].中国执业药师,2016, 13(3):23-26.
PAN M, XIE S L, QIU H Q, et al.Effect of alcohol detoxication prescription consisting of Semen hoveniae and Pueraria lobata at an optimal proportion on ethanol absorption and metabolism in humans[J].China Licensed Pharmacist, 2016, 13(3):23-26.
[12] GERLAI R, LAHAV M, GUO S, et al.Drinks like a fish: Zebra fish (Danio rerio) as a behavior genetic model to study alcohol effects[J].Pharmacology Biochemistry and Behavior,2000, 67(4):773-782.
[13] 崔佳琦, 彭桂英, 冯昊天, 等.茯苓-葛根-枳椇子混合药粉对斑马鱼解酒保肝作用研究[J].现代中药研究与实践, 2022, 36(6):24-28.
CUI J Q, PENG G Y, FENG H T, et al.Study on the anti-alcohol and hepatoprotective effect of Fuling-Gegen-zhijvzi mixed powder in zebrafish[J].Research and Practice on Chinese Medicines, 2022, 36(6):24-28.
[14] 戴文聪,刘莉,王坤元,等.急性酒精性脂肪肝斑马鱼模型的建立[J].临床肝胆病杂志,2013, 29(4):286-289.
DAI W C, LIU L, WANG K Y, et al.Establishment of a zebrafish model of alcohol-induced acute hepatic steatosis[J].Journal of Clinical Hepatology, 2013, 29(4):286-289.
[15] 李祥坤,曾亦菡.响应面优化霍山石斛百香果复合饮料工艺及抗运动疲劳研究[J].中国食品添加剂,2023, 34(1):272-281.
LI X K, ZENG Y H.Optimization of compound beverage of Dendrobium huoshanense and passion fruit by response surface method and its anti-fatigue effect[J].China Food Additives, 2023, 34(1):272-281.
[16] 张金桃. 响应面优化红酸汤固体饮料配方[J].粮食科技与经济, 2022, 47(4):109-113.
ZHANG J T.Optimization of red acid solid beverage formula by response surface[J].Food Science and Technology and Economy, 2022, 47(4):109-113.
[17] 辛世华,韩小珍.模糊数学评价结合响应面法优化马铃薯蛋糕加工工艺[J].食品工业科技,2019, 40(13):113-118;133.
XIN S H, HAN X Z.Optimization of potato cake processing technology based on fuzzy mathematic evaluation combination with response surface method[J].Science and Technology of Food Industry, 2019, 40(13):113-118;133.
[18] 葛炜炜,曹刚,吴育,等.还原型谷胱甘肽对酒精性脂肪肝斑马鱼的保肝作用研究[J].药物评价研究,2021, 44(8):1594-1599.
GE W W, CAO G, WU Y, et al.Liver protection effect of glutathione tablets on alcoholic fatty liver disease zebrafish[J].Drug Evaluation Research, 2021, 44(8):1594-1599.
[19] 赖裕玲. 虎杖苷对斑马鱼幼鱼酒精性脂肪肝的治疗作用及机制研究[D].广州:南方医科大学,2019.
LAI Y L.Therapeutical effects and mechanisms of polydatin on alcoholic fatty liver in larval zebrafish larvae[D].Guangzhou:Southern Medical University, 2019.
[20] PASSERI M J, CINAROGLU A, GAO C, et al.Hepatic steatosis in response to acute alcohol exposure in zebrafish requires sterol regulatory element binding protein activation[J].Hepatology, 2009, 49(2):443-452.
[21] 戴雨霖,郑飞,黄鑫,等.葛花人参配伍的解酒护肝机制[J].中国实验方剂学杂志, 2016, 22(4):45-49.
DAI Y L, ZHENG F, HUANG X, et al.Effect of compatibility of ginseng Radix et rhizoma and flower of Pueraria lobata on alleviating hangover and protecting liver[J].Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(4):45-49.
