Effect of replacing sulfur dioxide with hydroxytyrosol and chitooligosaccharide on quality of low-alcohol cider

  • YU Baihan ,
  • LIU Jialu ,
  • XU Binyan ,
  • YANG Xueshan ,
  • ZHU Xia
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2025-02-28

  Revised date: 2025-05-01

  Online published: 2026-01-22

Abstract

The addition of sulphur dioxide (SO2) in the fermentation of fruit wine can inhibit the deterioration caused by undesirable microorganisms, but it has potential safety risks to the health of consumers.To explore the effect of replacing SO2 with natural antibacterial agents on the quality of low-alcohol cider, Fuji apple juice was used as the raw material, and the different mass concentrations of hydroxytyrosol (HT, with purities of ≥98% and ≥20%) or chitooligosaccharide (COS, with a purity of ≥90%) were added before the alcoholic fermentation.The kinetics of Saccharomyces cerevisiae growth and reducing sugar consumption, as well as the physicochemical indexes, color and volatile aroma compounds of cider during fermentation were determined.In addition, sensory analysis was also conducted on the final samples.Results showed that compared with the group added with SO2, the alcoholic fermentation kinetics of cider added with different mass concentrations of HT or COS was significantly different.Among them, the addition of HT (≥20%) and SO2 treatment groups completed the fermentation on the 7th day, while the COS and HT (≥98%) treatment groups completed the fermentation on the 9th day.And the final reducing sugar mass concentrations of these samples was less than 4 g/L.The level of alcohol, pH value, total acidity and volatile acidity met the basic requirements of low-alcohol fruit wine.However, there were significant differences in total phenol content.Meanwhile, with the decrease of the purity and mass concentration of HT added in the cider samples, the chroma value of the cider samples gradually decreases, and the hue value increases accordingly.This change trend is the same as that of the COS group.In addition, the detection results of volatile compounds showed that the addition of both HT and COS before alcohol fermentation affected the aroma content of cider.Especially, the total content of volatile compounds in the cider samples added with 10 mg/L HT (≥98%) increased by 14.13% compared with the fermentation group added with SO2.Due to the increase in the content of floral and fruity volatile substances such as geraniol, amyl acetate, ethyl caprylate and ethyl decanoate, the sensory score of this cider sample reached 7.307 9.In summary, the addition of 10 mg/L HT (≥98%) can increase the content of volatile aroma compounds in cider, enrich the floral and fruity aroma of cider samples, and improve the taste and color of cider.

Cite this article

YU Baihan , LIU Jialu , XU Binyan , YANG Xueshan , ZHU Xia . Effect of replacing sulfur dioxide with hydroxytyrosol and chitooligosaccharide on quality of low-alcohol cider[J]. Food and Fermentation Industries, 2026 , 52(1) : 186 -197 . DOI: 10.13995/j.cnki.11-1802/ts.042561

References

[1] 陈青, 殷程凯, 郭自良, 等.基于改进YOLOv7的苹果生长状态及姿态识别[J].农业工程学报, 2024, 40(6):258-266.
CHEN Q, YIN C K, GUO Z L, et al.Apple growth status and posture recognition using improved YOLOv7[J].Transactions of the Chinese Society of Agricultural Engineering, 2024, 40(6):258-266.
[2] 彭彦昆, 孙晨, 刘乐, 等.苹果外部缺陷全表面在线检测分选装置研发[J].农业工程学报, 2022, 38(23):266-275.
PENG Y K, SUN C, LIU L, et al.Development of full-surface online detection and sorting device for external defects of apples[J].Transactions of the Chinese Society of Agricultural Engineering, 2022, 38(23):266-275.
[3] 杨超, 肖媚, 张菡, 等.苹果酒发酵过程中绿原酸胁迫对酿酒酵母生理特性的影响[J].中国食品学报, 2022, 22(1):116-124.
YANG C, XIAO M, ZHANG H, et al.Effects of chlorogenic acid stress on the physiological characteristics of Saccharomyces cerevisiae during the fermentation of cider[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(1):116-124.
[4] ESPARZA I, MARTÍNEZ-INDA B, CIMMINELLI M J, et al.Reducing SO2 doses in red wines by using grape stem extracts as antioxidants[J].Biomolecules, 2020, 10(10):1369.
[5] 魏广芬, 李梦递, 赵捷, 等.基于DFI-RSE电子鼻传感器阵列优化的葡萄酒SO2检测[J].农业工程学报, 2022, 38(7):291-299.
WEI G F, LI M D, ZHAO J, et al.Determination of SO2 in wine based on DFI-RSE electronic nose sensor array optimization[J].Transactions of the Chinese Society of Agricultural Engineering, 2022, 38(7):291-299.
[6] 陈晓姣, 刁体伟, 赖晓琴, 等.不同酿造阶段添加富含谷胱甘肽酵母衍生物对梨酒品质的影响[J].食品科学, 2023, 44(6):134-142.
CHEN X J, DIAO T W, LAI X Q, et al.Effect of addition of glutathione-enriched inactive dry yeast at different stages of brewing on the quality of pear wine[J].Food Science, 2023, 44(6):134-142.
[7] 冯帆, 姜醒睿, 王凌云, 等.没食子酸稳定‘户太八号’桃红葡萄酒香气与色泽[J].中国农业科学, 2024, 57(8):1592-1605.
FENG F, JIANG X R, WANG L Y, et al.The stabilization of aroma and color during Hutai-8 rose winemaking by gallic acid treatment[J].Scientia Agricultura Sinica, 2024, 57(8):1592-1605.
[8] NEHMÉ L, EL TEKLE M, BARAKAT N, et al.Alternative processes for apple juice stabilization and clarification:A bibliometric and comprehensive review[J].Processes, 2024, 12(2):296.
[9] YANG H, WANG S, CHEN M, et al.The Saccharomyces cerevisiae through increasing the expression of membrane transporter to produce more GSH inhibit the browning of pear wine[J].Food Bioscience, 2024, 58:103689.
[10] 马雷. 天然抑菌剂在葡萄酒酿造中的应用[D].大连:大连工业大学, 2017.
MA L.Application of natural antimicrobial agents in wine brewing[D].Dalian:Dalian Polytechnic University, 2017.
[11] MOTA C J A.Green routes to dimethyl carbonate:A green and versatile methylating reactant[J].Current Organic Chemistry, 2024, 28(14):1069-1078.
[12] NADERI-SAMANI E, RAZAVI R S, NEKOUEE K, et al.Synthesis of silver nanoparticles for use in conductive inks by chemical reduction method[J].Heliyon, 2023, 9(10):e20548.
[13] DOMÍNGUEZ-PERLES R, AUÑÓN D, FERRERES F, et al.Physiological linkage of gender, bioavailable hydroxytyrosol derivatives, and their metabolites with systemic catecholamine metabolism[J].Food & Function, 2017, 8(12):4570-4581.
[14] TERRACINA S, PETRELLA C, FRANCATI S, et al.Antioxidant intervention to improve cognition in the aging brain:The example of hydroxytyrosol and resveratrol[J].International Journal of Molecular Sciences, 2022, 23(24):15674.
[15] NOGUERA-NAVARRO C, MONTORO-GARCÍA S, ORENES-PIÑERO E.Hydroxytyrosol:Its role in the prevention of cardiovascular diseases[J].Heliyon, 2023, 9(1):e12963.
[16] 李春燕. 油橄榄叶中羟基酪醇的提取及橄榄酒的研制[D].成都:西华大学, 2014.
LI C Y.Research on hydroxytyrosol extraction form olive leaves and olive wine brewing[D].Chengdu:Xihua University, 2014.
[17] RAPOSO R, RUIZ-MORENO M J, GARDE-CERDÁN T, et al.Effect of hydroxytyrosol on quality of sulfur dioxide-free red wine[J].Food Chemistry, 2016, 192:25-33.
[18] 郝振铭, 孙珍.壳寡糖对葡萄酒的抗氧化和抑菌作用[J].现代食品科技, 2019, 35(12):216-224;60.
HAO Z M, SUN Z.Antioxidant and antibacterial effects of wine added with chitooligosaccharide[J].Modern Food Science & Technology, 2019, 35(12):216-224;60.
[19] 刁体伟, 陈晓姣, 赖晓琴.壳聚糖及其衍生物在果酒中应用的研究进展[J].食品工业科技, 2022, 43(6):467-473.
DIAO T W, CHEN X J, LAI X Q.Research progress on application of chitosan and its derivatives in fruit wine[J].Science and Technology of Food Industry, 2022, 43(6):467-473.
[20] 吴霞, 孟掉琴, 岳田利, 等.血脂干预型复合苹果汁果实品种筛选与果汁品质评价[J].中国食品学报, 2020, 20(8):286-294.
WU X, MENG D Q, YUE T L, et al.Variety screening and fruit juice quality evaluation of lipid-interfered compound apple juice[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(8):286-294.
[21] 周得刚, 徐彬艳, 王青霞, 等.戴尔有孢圆酵母与酿酒酵母细胞间接触对‘赤霞珠’葡萄酒风味品质的影响[J].中国农业科学, 2024, 57(16):3264-3282.
ZHOU D G, XU B Y, WANG Q X, et al.Effects of cell-to-cell contact between Torulaspora delbrueckii and Saccharomyces cerevisiae on the flavor and quality of Cabernet Sauvignon wine[J].Scientia Agricultura Sinica, 2024, 57(16):3264-3282.
[22] LIANG Z J, ZHANG P Z, MA W, et al.Physicochemical properties, antioxidant activities and comprehensive phenolic profiles of tea-macerated Chardonnay wine and model wine[J].Food Chemistry, 2024, 436:137748.
[23] 袁倩, 李嘉欣, 柳秉红, 等.不同酵母多糖添加条件下早酥梨-美乐低醇复合果酒品质研究[J].农业机械学报, 2024, 55(5):419-430.
YUAN Q, LI J X, LIU B H, et al.Quality of low-alcohol Zaosu pear-merlot wine under different yeast polysaccharide addition conditions[J].Transactions of the Chinese Society for Agricultural Machinery, 2024, 55(5):419-430.
[24] 藏伟, 刘叶, 刘宇, 等.本土季也蒙毕赤酵母在干红葡萄酒中试生产中的应用潜力[J].食品科学, 2023, 44(18):117-125.
ZANG W, LIU Y, LIU Y, et al.Application potential of native Meyerozyma guilliermondii in pilot production of dry red wine[J].Food Science, 2023, 44(18):117-125.
[25] 祝霞, 赵丹丹, 李俊娥, 等.河西走廊产区酒球菌酯酶活性对葡萄酒酯类香气物质的影响[J].农业工程学报, 2021, 37(1):315-322.
ZHU X, ZHAO D D, LI J E, et al.Effects of esterase activity of alcoholicus in Hexi Corridor production areas on ester aroma compounds in wine[J].Transactions of the Chinese Society of Agricultural Engineering, 2021, 37(1):315-322.
[26] YANG H, SUN J Y, TIAN T T, et al.Physicochemical characterization and quality of Dangshan pear wines fermented with different Saccharomyces cerevisiae[J].Journal of Food Biochemistry, 2019, 43(8):e12891.
[27] HAO Z M, ZHANG Y R, SUN Z, et al.Chitooligosaccharide as a possible replacement for sulfur dioxide in winemaking[J].Applied Sciences, 2020, 10(2):578.
[28] RAPOSO R, RUIZ-MORENO M J, GARDE-CERDÁN T, et al.Replacement of sulfur dioxide by hydroxytyrosol in white wine:Influence on both quality parameters and sensory[J].LWT, 2016, 65:214-221.
[29] MARTÍNEZ J A, MELGOSA M, PÉREZ M M, et al.Note.visual and instrumental color evaluation in red wines[J].Food Science and Technology International, 2001, 7(5):439-444.
[30] 陈鑫龙, 张媛媛, 徐令斌, 等.用多酚类指标表征干红葡萄酒味感质量的模型构建[J].农业工程学报, 2024, 40(8):281-289.
CHEN X L, ZHANG Y Y, XU L B, et al.Model construction for characterizing the flavor quality of dry red wine based on polyphenolic indexes[J].Transactions of the Chinese Society of Agricultural Engineering, 2024, 40(8):281-289.
[31] ZHAI X C, FENG Y X, ZHAO R J, et al.Nisin as a safe and effective alternative to sulfur dioxide in enhancing dateplum persimmon (Diospyros lotus L.) wine quality[J].LWT, 2024, 193:115774.
[32] CASTRO-MARÍN A, BUGLIA A G, RIPONI C, et al.Volatile and fixed composition of sulphite-free white wines obtained after fermentation in the presence of chitosan[J].LWT, 2018, 93:174-180.
[33] JIANG X H, LU Y Y, LIU S Q.Effects of pectinase treatment on the physicochemical and oenological properties of red dragon fruit wine fermented with Torulaspora delbrueckii[J].LWT, 2020, 132:109929.
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