When cyclodextrin (CD) is produced by cyclodextrin glucosyltransferase (CGTase), some starch substrates cannot be used, which leads to low utilization rate of starch raw materials and low production efficiency.To solve the above problems, a new starch debranching enzyme, glycogen debranching enzyme CgGDE from Candida glabrata, was cloned and expressed by Escherichia coli BL21(DE3).When the fermentation temperature was 25 ℃, the final concentration of the inducer IPTG was 0.1 mmol/L, and the induction time was 36 h, the activity of CgGDE reached 69.3 U/mL.The results of enzymatic properties showed that the optimum pH of CgGDE was 6.0, the optimum temperature was 35 ℃.β-CD was prepared by combining CgGDE with β-CGTase.Results showed that under non-solvent conditions, when the concentration of potato starch was 50 g/L, at 40 ℃, pH was 6.0, the CgGDE addition was 750 U/g dry starch, the β-CGTase addition was 7 U/g dry starch, the total yield of β-CD reached 53.1% after 24 h, which was 93.8% and 50.9% higher than that transformation with β-CGTase alone and combination of CGTase enzyme and isoamylase.This work provides a basis for the transformation of β-CD under non-solvent conditions.
HUANG Jingjia
,
TAO Xiumei
,
KONG Deming
,
CHEN Xingyi
,
WU Jing
,
CHEN Sheng
,
WANG Lei
. Enhancing β-cyclodextrin production by combining glycogen debranching enzyme and cyclodextrin glucantransferase[J]. Food and Fermentation Industries, 2026
, 52(1)
: 244
-250
.
DOI: 10.13995/j.cnki.11-1802/ts.042280
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