In response to the issues of narrow antibacterial spectrum, insufficient biological safety and poor environmental stability of antimicrobial peptides in food preservation applications, this study adopted a molecular hybridization strategy to hybridize Cecropin A (1-8) with Aurein 1.2 (6-13), and through amino acid substitution optimization, successfully constructed a novel hybrid antimicrobial peptide CA16-L.Subsequently, the antibacterial activity, biocompatibility, and stability of CA16-L were systematically evaluated, and its mechanism of action and preservative performance were deeply explored.Results showed that the cell selectivity index (SIall) of CA16-L was 59.26, and it exhibited a strong killing effect on common foodborne pathogenic bacteria.Even under different salt ion, high temperature, and extreme pH conditions, CA16-L can still maintained strong antibacterial activity.Mechanism studies indicated that CA16-L exerted antibacterial effects by disrupting bacterial cell membranes and interfering with DNA replication, effectively reducing the risk of drug resistance.The fresh pork preservation model confirmed that CA16-L could effectively inhibit the growth of Staphylococcus aureus ATCC29213, extend the shelf life of meat products, and had good preservative performance.This study provides a new idea for the development of efficient and safe food preservatives and is of great significance for promoting the sustainable development of the food industry.
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