Untargeted metabolomics investigation of the protective effects of raspberry crude polysaccharides against UVB-induced skin photodamage in mice

  • WANG Yuting ,
  • YANG Yongjing ,
  • HAN Le ,
  • JI Yiming ,
  • CHE Guangxue
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  • (School of Ecological and Environmental Engineering, Qinghai University, Xining 810000, China)

Received date: 2025-01-22

  Revised date: 2025-07-03

  Online published: 2026-03-25

Abstract

This study employed untargeted metabolomics to investigate the protective mechanisms of raspberry crude polysaccharides (RCP) against ultraviolet B (UVB)-induced skin photodamage.A UVB-induced skin photodamage model was established in BALB/c mice.Histopathological changes in skin tissues were assessed using hematoxylin-eosin staining.Superoxide dismutase (SOD) and catalase (CAT) activities in skin tissues were quantified via microplate assays, while IL-6, TNF-α, and matrix metalloproteinase-3 (MMP-3) levels in skin tissues were determined by enzyme-linked immunosorbent assay. Skin metabolic profiles were analyzed by untargeted metabolomics.Results demonstrated that RCP alleviated UVB-induced epidermal thickening, hyperkeratosis, fibrous tissue hyperplasia, and inflammatory cell infiltration.RCP intervention significantly enhanced SOD and CAT activities while reducing IL-6, TNF-α, and MMP-3 levels in UVB-damaged skin.Untargeted metabolomics identified 58 differentially expressed metabolites in skin tissues, with pathway enrichment analysis revealing alanine, aspartate, and glutamate metabolism as the most significantly enriched amino acid pathway.Correlation analysis indicated a strong positive association between alanine and SOD activity, whereas DL-asparagine, glutamate, L-glutamine, and N-acetyl-L-aspartate exhibited negative correlations with SOD.These findings suggest that RCP mitigates UVB-induced skin oxidative stress by modulating alanine, aspartate, and glutamate metabolism.

Cite this article

WANG Yuting , YANG Yongjing , HAN Le , JI Yiming , CHE Guangxue . Untargeted metabolomics investigation of the protective effects of raspberry crude polysaccharides against UVB-induced skin photodamage in mice[J]. Food and Fermentation Industries, 2026 , 52(5) : 91 -100 . DOI: 10.13995/j.cnki.11-1802/ts.042227

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