Food and Fermentation Industries >

2026 , Vol. 52 >Issue 5: 121 - 131

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044477

Mechanism of the effect of postbiotic from Bifidobacterium longum subsp. longum HSBL001 on colitis based on integrated metagenomics and metabolomics

  • LIU Jianbing ,
  • CHENG Hao ,
  • XIONG Yanxia ,
  • CHEN Huijun ,
  • YIN Lili ,
  • MEI Liya ,
  • ZHANG Yan ,
  • XIE Youfa ,
  • ZHANG Yang
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  • 1(Jiangzhong Pharmaceutical Co. Ltd., Nanchang 330077, China)
    2(Jincheng Haisi Pharmaceutical Co. Ltd., Jincheng 048006, China)

Received date: 2025-09-10

  Revised date: 2025-10-20

  Online published: 2026-03-25

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Abstract

Postbiotics are inactivated microorganisms or included their metabolites that benefit host health.Compared with probiotics, they have higher safety, stronger stability, and a broader range of action targets, thus showing significant advantages in alleviating inflammatory bowel disease.This study evaluated the ameliorative effect of the postbiotic from Bifidobacterium longum subsp. longum HSBL001 on dextran sulfate sodium -induced ulcerative colitis mouse model, and explored the potential mechanism of this effect via metagenomics and metabolomics.Results indicate that HSBL001 significantly alleviates colitis-related symptoms, including reducing the disease activity index, increasing colon length, improving histopathological damage, regulating inflammatory cytokine secretion (increasing IL-10 while decreasing IL-6 and TNF-α), and alleviating splenomegaly.Metagenomic analysis showed that HSBL001 significantly increased the abundance of beneficial bacteria such as Duncaniella dubosii, Faecalibacterium rodentium, and Bacteroides thetaiotaomicron, while reducing the abundance of bacteria like Mucispirillum schaedleri and Pumilibacter muris. Metabolomic analysis revealed that HSBL001 significantly upregulated 95 metabolites compared to the model group, including S-nitrosoglutathione, α-linolenic acid, and 7α-hydroxy-3-oxocholest-4-en-27-oic acid, while significantly downregulating 80 metabolites including 3-hydroxyisovaleric acid and N-Acetyl-D-glucosaminitol.Pathway enrichment analysis of these differential metabolites shows that the postbiotic alleviates colitis mainly by regulating pathways such as primary bile acid biosynthesis and α-linolenic acid metabolism.In conclusion, supplementation with the HSBL001 effectively alleviates ulcerative colitis symptoms in mice.Its potential mechanisms are closely linked to regulating gut microbiota composition, increasing the abundance of bacteria such as D.dubosii and F.rodentium, as well as modulating primary bile acid biosynthesis and α-linolenic acid metabolism.This study provides a new intervention method and research insights for alleviating colitis, and a theoretical basis for the industrial application of postbiotics.

Key words： dextran sodium sulfate; inflammatory factors; bile acid metabolism; ulcerative colitis; milk-based postbiotic elements

Cite this article

LIU Jianbing , CHENG Hao , XIONG Yanxia , CHEN Huijun , YIN Lili , MEI Liya , ZHANG Yan , XIE Youfa , ZHANG Yang . Mechanism of the effect of postbiotic from Bifidobacterium longum subsp. longum HSBL001 on colitis based on integrated metagenomics and metabolomics[J]. Food and Fermentation Industries, 2026 , 52(5) : 121 -131 . DOI: 10.13995/j.cnki.11-1802/ts.044477

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