This study successfully prepared Macrobrachium rosenbergii oil-high internal phase emulsion gels (MRO-HIPEs) using soy protein isolate microgel particles-gum arabic (SPIMPs-GA) complexes as emulsifiers.The effects of GA concentration (0-4%) on the physicochemical and 3D printing properties of MRO-HIPEs were systematically investigated.Comprehensive characterization was performed through confocal laser scanning microscopy (CLSM), texture profile analysis, rheological measurements, and 3D printing assessment.Results demonstrated that electrostatic interactions between GA and SPIMPs created dense interfacial films that effectively prevented droplet aggregation.Increasing the GA concentration to 4% significantly enhanced the oil-holding capacity (96.21%), thermal stability (89.15%), and freeze-thaw stability (85.55%) of MRO-HIPEs.Texture analysis indicated GA can strengthen mechanical properties of MRO-HIPEs, while rheological tests revealed its role in boosting elastic modulus and improving flow characteristics.Furthermore, MRO-HIPEs containing 4% GA exhibited the optimal performance, particularly in maintaining excellent 3D printing shape fidelity.These findings establish a solid theoretical foundation for applying MRO-HIPEs in food gel systems, while providing practical technical support for developing stable emulsion-based products with tunable functional properties.
LI Mengmeng
,
HAO Guijie
,
LU Yuanchao
,
YU Ningxiang
,
MENG Xianghe
. Effects of gum arabic on physicochemical and 3D printing properties of Macrobrachium rosenbergii oil high internal phase emulsion gels[J]. Food and Fermentation Industries, 2026
, 52(6)
: 90
-96
.
DOI: 10.13995/j.cnki.11-1802/ts.043593
[1] SHI A M, FENG X Y, WANG Q, et al.Pickering and high internal phase Pickering emulsions stabilized by protein-based particles:A review of synthesis, application and prospective[J].Food Hydrocolloids, 2020, 109:106117.
[2] ARAIZA-CALAHORRA A, WANG Y Q, BOESCH C, et al.Pickering emulsions stabilized by colloidal gel particles complexed or conjugated with biopolymers to enhance bioaccessibility and cellular uptake of curcumin[J].Current Research in Food Science, 2020, 3:178-188.
[3] REHMAN A, LIANG Q F, KARIM A, et al.Pickering high internal phase emulsions stabilized by biopolymeric particles:From production to high-performance applications[J].Food Hydrocolloids, 2024, 150:109751.
[4] GUO Z A, LI Z H, CEN S Y, et al.Preparation of Pangasius hypophthalmus protein-stabilized Pickering emulsions and 3D printing application[J].Journal of Food Engineering, 2023, 341:111333.
[5] AKGONULLU D Z, O’HAGAN N M, MURRAY B S, et al.Bulk and interfacial behavior of potato protein-based microgels[J].Langmuir, 2024, 40(41):21341-21351.
[6] 贾立奥, 姚飞, 寇锟, 等.高内相Pickering乳液的稳定性及在食品3D打印中的应用[C].中国食品科学技术学会第二十一届年会论文摘要集.重庆, 2024:761-762.
JIA L A, YAO F, KOU K, et al.Stability of high internal phase Pickering emulsion and its application in food 3D printing[C].Abstracts of the 21st Annual Meeting of CIFST.Chongqing, 2024:761-762.
[7] ZHANG S N, HOLMES M, ETTELAIE R, et al.Pea protein microgel particles as Pickering stabilisers of oil-in-water emulsions:Responsiveness to pH and ionic strength[J].Food Hydrocolloids, 2020, 102:105583.
[8] SHEN S K, CHEN Y W, YU W T, et al.High internal phase Pickering emulsions stabilized by modified sturgeon myofibrillar protein for quercetin delivery[J].Food Hydrocolloids, 2023, 144:108926.
[9] GAO T, WU X X, GAO Y T, et al.Construction of emulsion gel based on the interaction of anionic polysaccharide and soy protein isolate:Focusing on structural, emulsification and functional properties[J].Food Chemistry, 2024, 22:101377.
[10] KARBASI M, ASKARI G.Modification of whey protein microgel particles with mono-oligo-and polysaccharides through the Maillard reaction:Effects on structural and techno-functional properties[J].Food Structure, 2021, 28:100184.
[11] LAKKIS J, VILLOTA R.Effect of acylation on substructural properties of proteins:A study using fluorescence and circular dichroism[J].Journal of Agricultural and Food Chemistry, 1992, 40(4):553-560.
[12] DICKINSON E.Hydrocolloids at interfaces and the influence on the properties of dispersed systems[J].Food Hydrocolloids, 2003, 17(1):25-39.
[13] LIU X, GUO J, WAN Z L, et al.Wheat gluten-stabilized high internal phase emulsions as mayonnaise replacers[J].Food Hydrocolloids, 2018, 77:168-175.
[14] SUN C H, GUNASEKARAN S.Effects of protein concentration and oil-phase volume fraction on the stability and rheology of menhaden oil-in-water emulsions stabilized by whey protein isolate with xanthan gum[J].Food Hydrocolloids, 2009, 23(1):165-174.
[15] LI M, FENG L, DAI Z Q, et al.Improvement of 3D printing performance of whey protein isolate emulsion gels by regulating rheological properties:Effect of polysaccharides incorporation[J].Food and Bioprocess Technology, 2025, 18(1):633-647.
[16] LIU W, GAO H X, MCCLEMENTS D J, et al.Stability, rheology, and β-carotene bioaccessibility of high internal phase emulsion gels[J].Food Hydrocolloids, 2019, 88:210-217.
[17] GHOSH S, COUPLAND J N.Factors affecting the freeze-thaw stability of emulsions[J].Food Hydrocolloids, 2008, 22(1):105-111.
[18] OLIVER C M, KHER A, MCNAUGHTON D, et al.Use of FTIR and mass spectrometry for characterization of glycated caseins[J].The Journal of Dairy Research, 2009, 76(1):105-110.
[19] 陆元超, 杨骥, 叶沁, 等.山茶油微胶囊的制备表征及其在饼干中的应用研究[J].食品与发酵工业, 2025, 51(10):17-22.
LU Y C, YANG J, YE Q, et al.Preparation and characterization of Camellia oil microcapsules and their application in biscuits[J].Food and Fermentation Industries, 2025, 51(10):17-22.
[20] WANG H X, HU L D, DU J, et al.Development of rheologically stable high internal phase emulsions by gelatin/chitooligosaccharide mixtures and food application[J].Food Hydrocolloids, 2021, 121:107050.
[21] ZUO Z Y, ZHANG X X, LI T, et al.High internal phase emulsions stabilized solely by sonicated quinoa protein isolate at various pH values and concentrations[J].Food Chemistry, 2022, 378:132011.
[22] XU B G, WANG X D, CHITRAKAR B, et al.Effect of various physical modifications of pea protein isolate (PPI) on 3D printing behavior and dysphagia properties of strawberry-PPI gels[J].Food Hydrocolloids, 2025, 158:110498.