Edible cling film is widely used in fruit and vegetable preservation and has important commercial value.This study detailed the development of an edible film using gelatin and glycerol as a matrix to immobilize Lactiplantibacillus plantarum SCSL-1 for food preservation.The initial step involved quantifying the viable bacterial count in the prepared film.Subsequently, the mechanical properties, moisture content, swelling rate, water solubility, water vapor permeability, and light transmittance of the film were optimized.Structural analysis of the edible film was performed using Fourier transform infrared spectroscopy and scanning electron microscopy.Finally, the efficacy of the film was evaluated through its application in preserving mangoes.The application in mango preservation was also evaluated.The results showed that the viable bacterial count remained above 107 CFU/g in the gelatin film containing 1.4 g glycerol after storage at 5 ℃ for 14 days.The L.plantarum SCSL-1 edible film was able to effectively slow down the rotting and deterioration of mango, to maintain its freshness and to significantly reduce the bacterial growth rate in mango preservation and storage.In conclusion, the L.plantarum SCSL-1 edible cling film shows great potential for the packaging and preservation of perishable fruits and vegetables.
XU Yuheng
,
LI Ke
. Preparation, characterization, and application of edible cling film loaded with lactic acid bacteria[J]. Food and Fermentation Industries, 2026
, 52(6)
: 310
-316
.
DOI: 10.13995/j.cnki.11-1802/ts.044591
[1] ZHANG Y L, CUI Q L, WANG Y, et al. Effect of carboxymethyl chitosan-gelatin-based edible coatings on the quality and antioxidant properties of sweet cherry during postharvest storage[J]. Scientia Horticulturae, 2021, 289:110462.
[2] KINGWASCHARAPONG P, ARISA K, KARNJANAPRATUM S, et al. Effect of gelatin-based coating containing frog skin oil on the quality of persimmon and its characteristics[J]. Scientia Horticulturae, 2020, 260:108864.
[3] LÓPEZ DE LACEY A M, LÓPEZ-CABALLERO M E, GÓMEZ-ESTACA J, et al. Functionality of Lactobacillus acidophilus and Bifidobacterium bifidum incorporated to edible coatings and films[J]. Innovative Food Science & Emerging Technologies, 2012, 16:277-282.
[4] TEMIZ N N, ÖZDEMIR K S. Microbiological and physicochemical quality of strawberries (Fragaria×Ananassa) coated with Lactobacillus rhamnosus and inulin enriched gelatin films[J]. Postharvest Biology and Technology, 2021, 173:111433.
[5] LIU W X, WANG J J, XIAO X K, et al. Antimicrobial effects and metabolomics analysis of cell-free supernatant produced by Pediococcus acidilactici LWX 401 isolated from Yunnan traditional pickles[J]. LWT, 2024, 191:115626.
[6] SUN L J, SUN J J, CHEN L, et al. Preparation and characterization of chitosan film incorporated with thinned young apple polyphenols as an active packaging material[J]. Carbohydrate Polymers, 2017, 163:81-91.
[7] ZHAO G Y, ZHOU C Y, FAN F Y. Preparation and properties of soy protein isolate/cotton-nanocrystalline cellulose films[J]. International Journal of Polymer Science, 2021, 2021:5518136.
[8] KHODAEI D, HAMIDI-ESFAHANI Z, LACROIX M. Gelatin and low methoxyl pectin films containing probiotics: Film characterization and cell viability[J]. Food Bioscience, 2020, 36:100660.
[9] LIU F, CHIOU B S, AVENA-BUSTILLOS R J, et al. Study of combined effects of glycerol and transglutaminase on properties of gelatin films[J]. Food Hydrocolloids, 2017, 65:1-9.
[10] SHAHRAMPOUR D, KHOMEIRI M, ALI RAZAVI S M, et al. Development and characterization of alginate/pectin edible films containing Lactobacillus plantarum KMC 45[J]. LWT, 2020, 118:108758.
[11] KHODAEI D, HAMIDI-ESFAHANI Z. Influence of bioactive edible coatings loaded with Lactobacillus plantarum on physicochemical properties of fresh strawberries[J]. Postharvest Biology and Technology, 2019, 156:110944.
[12] BERTUZZI M A, CASTRO VIDAURRE E F, ARMADA M, et al. Water vapor permeability of edible starch based films[J]. Journal of Food Engineering, 2007, 80(3):972-978.
[13] BANDEIRA S F, DE SOUZA GOMES DA SILVA R, DE MOURA J M, et al. Modified gelatin films from croaker skins: Effects of pH, and addition of glycerol and chitosan[J]. Journal of Food Process Engineering, 2015, 38(6):613-620.
[14] GIALAMAS H, ZINOVIADOU K G, BILIADERIS C G, et al. Development of a novel bioactive packaging based on the incorporation of Lactobacillus sakei into sodium-caseinate films for controlling Listeria monocytogenes in foods[J]. Food Research International, 2010, 43(10):2402-2408.
[15] SETTIER-RAMÍREZ L, LÓPEZ-CARBALLO G, GAVARA R, et al. Antilisterial properties of PVOH-based films embedded with Lactococcus lactis subsp. lactis[J]. Food Hydrocolloids, 2019, 87:214-220.
[16] MA D H, JIANG Y, AHMED S, et al. Physical and antimicrobial properties of edible films containing Lactococcus lactis[J]. International Journal of Biological Macromolecules, 2019, 141:378-386.
[17] AYAD SHAMSALLAH A, OMER RASHID S. Development in gelatin-matrix composite films: The incorporation of vitamin C adducts enhances the optical behaviors of gelatin films[J]. Arabian Journal of Chemistry, 2024, 17(2):105541.
[18] LI S Y, MA Y L, JI T T, et al. Cassava starch/carboxymethylcellulose edible films embedded with lactic acid bacteria to extend the shelf life of banana[J]. Carbohydrate Polymers, 2020, 248:116805.
[19] GUIMARÃES A, ABRUNHOSA L, PASTRANA L M, et al. Edible films and coatings as carriers of living microorganisms: A new strategy towards biopreservation and healthier foods[J]. Comprehensive Reviews in Food Science and Food Safety, 2018, 17(3):594-614.
[20] YANG Z M, GUAN C, ZHOU C, et al. Amphiphilic chitosan/carboxymethyl gellan gum composite films enriched with mustard essential oil for mango preservation[J]. Carbohydrate Polymers, 2023, 300:120290.
[21] HASHEMI S M B, JAFARPOUR D. Bioactive edible film based on Konjac glucomannan and probiotic Lactobacillus plantarum strains: Physicochemical properties and shelf life of fresh-cut kiwis[J]. Journal of Food Science, 2021, 86(2):513-522.
