To explore the optimal high-density cultivation conditions of Bifidobacterium animalis subsp.lactis IMAU12344 isolated from breast milk in Inner Mongolia, the composition of high-density fermentation medium and static culture conditions were optimized on the basis of modified MRS medium by single-factor experiment, compound experiment, Plackett-Burman experiment, steepest climbing experiment, and response surface experiment design.Results showed that the optimum medium formula for the strain was lactose 59.56 g/L, yeast peptone 60.76 g/L, disodium hydrogen phosphate 14.32 g/L, monosodium phosphate 6.24 g/L, MnSO4·5H2O 0.16 g/L, L-cysteine hydrochloride 0.5 g/L, and Tween-80 1 mL/L.The optimum static culture conditions were as follows:inoculation amount 2% (volume fraction), initial pH 6.5, and culture temperature 37 ℃.Under these conditions, the viable count cultured with the optimal medium formula could reach (4.50±0.05)×109 CFU/mL, which was 3.06 times higher than that of the modified MRS medium.By utilizing the existing high-density fermentation process for B.animalis subsp.lactis, the viable count could reach (1.51±0.14)×1010 CFU/mL, which was 12.60 times higher than that of the modified MRS medium.In this study, the optimal medium formula and static cultivation conditions for high-density fermentation of B.animalis subsp.lactis IMAU12344 were determined, achieving an improvement in fermentation efficiency, and providing parameter support for its industrial application.
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