Food and Fermentation Industries >

2026 , Vol. 52 >Issue 8: 188 - 199

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044064

Study on ameliorative effects of compound Lactiplantibacillus plantarum on hyperlipidemia and gut microbiota dysbiosis in rats

  • LIU Xuelian ,
  • SUN Xin ,
  • BAHEBAN Yeerjiang ,
  • KUERDAN Hudayibieergen ,
  • REZIYA Bieerdimulati ,
  • XINHUA Nabi
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China)
    2(Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China)
    3(Srational for Drug Control and Medical Device Varification of Xinjiang Military Command, Urumqi 830000, China)

Received date: 2025-07-29

  Revised date: 2025-11-21

  Online published: 2026-05-07

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Abstract

Traditional fermented mare milk from Xinjiang has a long history of folk use, contains a diverse community of beneficial microorganisms.These microorganisms contribute to the regulation of blood lipid levels and the mitigation of hyperlipidemia.This study investigated four strains of Lactiplantibacillus spp.isolated from Xinjiang fermented mare milk, evaluated their in vitro colonization properties, and developed a composite probiotic formulation.By establishing a hyperlipidemia rat model, this study detected serum biochemical indicators, liver pathology and gut microbiota diversity and composition, to evaluate the lipid-lowering effects of the probiotic and its impact on gut microbiota and the underlying mechanisms.Results showed that the composite probiotic significantly reduced body weight gain (P<0.01) and serum levels of total cholesterol and low-density lipoprotein cholesterol (P<0.01).It also improved the liver index and alleviated hepatic lipid deposition.Metagenomic analysis revealed that a high-fat diet induces gut microbiota dysbiosis.At the phylum level, the model group exhibits an increased Bacillota/Bacteroidota ratio, whereas all treatment groups showed a reduced ratio.At the genus level, the composite probiotic group up-regulated the abundances of Lactobacillus, Ruminococcus, and Oscillibacter.Correlation analysis indicated that Blautia_sp.was positively correlated with TC, triglyceride, and bile acids (P<0.05), while negatively correlated with short-chain fatty acids (SCFAs) (P<0.05).Conversely, Lactobacillus intestinalis was negatively correlated with bile acids (P<0.05) and positively correlated with SCFAs (P<0.05).Based on gut microbiota functional analysis, probiotics significantly regulated metabolic pathways related to the endocrine system and cardiovascular diseases (P<0.05).Additionally, the composite probiotic increased acetic acid and butyric acid levels in feces and reduced serum bile acid content (P<0.01).Concurrently, four Lactiplantibacillus spp.strains exhibited strong self-aggregation ability (24 h>90%), with surface hydrophobicity tests indicating high hydrophobicity to xylene (23.38%-31.59%).Their adhesion rates to Caco-2 cells ranged from 2.66% to 10.00%, suggesting good potential for intestinal colonization to facilitate probiotic functions.This indicates that these strains possess a certain potential for intestinal colonisation, which may facilitate their probiotic functions within the host.In summary, the composite probiotic effectively ameliorates hyperlipidemia and related metabolic disorders by modulating the gut microbiota structure and metabolic function.

Key words： compound probiotics; probiotic characteristics; hyperlipidemia; gut microbiota; metagenomics

Cite this article

LIU Xuelian , SUN Xin , BAHEBAN Yeerjiang , KUERDAN Hudayibieergen , REZIYA Bieerdimulati , XINHUA Nabi . Study on ameliorative effects of compound Lactiplantibacillus plantarum on hyperlipidemia and gut microbiota dysbiosis in rats[J]. Food and Fermentation Industries, 2026 , 52(8) : 188 -199 . DOI: 10.13995/j.cnki.11-1802/ts.044064

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