Food and Fermentation Industries >

2026 , Vol. 52 >Issue 8: 238 - 245

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043908

Screening, mutagenesis, and fermentation process optimization of high-yielding pyrroloquinoline quinone strains

  • ZHENG Xiaoxian ,
  • WANG Hailong ,
  • ZHAN Qing ,
  • ZHOU Arong ,
  • JIE Xiaoling ,
  • LIN Mengdan ,
  • CHEN Xiuming ,
  • FANG Dongsheng
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  • (Fujian Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China)

Received date: 2025-07-15

  Revised date: 2025-09-12

  Online published: 2026-05-07

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Abstract

Pyrroloquinoline quinone (PQQ) plays an important role in the fields of medicine, functional foods, cosmetics, and modern agriculture, with microbial fermentation being its economical and efficient production method.High-yielding PQQ strains in this study were screened to provide theoretical and technical support for industrial production.The research adopted a strategy combining high-throughput screening, spectrophotometric detection, and HPLC validation to isolate PQQ-producing strains from environmental wastewater.The strain with the highest yield was selected for 16S rDNA sequencing to determine its taxonomic position.Fermentation culture conditions for the strain were optimized, and mutagenesis was performed using atmospheric and room temperature plasma (ARTP) technology.Fermentation process studies were conducted in a 20 L fermenter.Results showed that a PQQ-producing strain FQ00818 with a yield of 25.6 mg/L was screened, and it was identified as Hyphomicrobium denitrificans.Through optimization of fermentation conditions, the PQQ yield of this strain increased to 41.2 mg/L.Using ARTP mutagenesis, a high-producing PQQ mutant strain FQ00818-31 was obtained with a shake-flask yield of 51.2 mg/L.In a 20 L fermenter, using a dynamic methanol fed-batch strategy, PQQ production increased to 772 mg/L.The strain screened in this study showed considerable industrial potential, and the rapid high-throughput screening method provided serves as a reference for the screening of other high-yielding PQQ strains.

Key words： pyrroloquinoline quinone; Hyphomicrobium denitrificans; high-throughput screening; atmospheric and room temperature plasma; Methylotrophic bacteria

Cite this article

ZHENG Xiaoxian , WANG Hailong , ZHAN Qing , ZHOU Arong , JIE Xiaoling , LIN Mengdan , CHEN Xiuming , FANG Dongsheng . Screening, mutagenesis, and fermentation process optimization of high-yielding pyrroloquinoline quinone strains[J]. Food and Fermentation Industries, 2026 , 52(8) : 238 -245 . DOI: 10.13995/j.cnki.11-1802/ts.043908

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