Iron deficiency anemia (IDA) is a prevalent complication during pregnancy, posing significant risks to maternal and fetal health.This study established a rat model of gestational IDA and investigated the therapeutic effects and underlying mechanisms of donkey-hide gelatin oligopeptides (DGOP).A low-iron diet successfully induced gestational IDA in rats.The animals were divided into six groups:model control, conventional iron supplement control, three DGOP intervention groups (low, medium, and high doses), and one combination group (conventional iron plus low-dose DGOP).Comprehensive measurements included blood routine parameters, serum iron metabolism markers, trace element levels, liver and kidney function, inflammatory and oxidative stress indicators, and pregnancy outcomes.Results demonstrated that DGOP interventions, particularly at medium and high doses, and the combination treatment significantly increased hemoglobin levels and improved anemia.These treatments also regulated key iron metabolism indicators and reduced pro-inflammatory cytokine levels.The study observed no significant effects on liver or kidney function or oxidative stress markers.DGOP exhibits significant efficacy in ameliorating gestational IDA, with its mechanism related to improved iron metabolism and reduced inflammation.These findings identify 300 mg/kg donkey-hide gelatin oligopeptides combined with 5.43 mg/kg ferrous sulfate as the optimal dosage for treating gestational iron deficiency anemia.The study supports donkey-hide gelatin oligopeptides’ potential as a dietary intervention for managing IDA during pregnancy.
LIU Xinran
,
LIU Peng
,
GUO Qianying
,
FAN Rui
,
LI Yong
,
LI Di
. Ameliorative effect of donkey-hide gelatin oligopeptides on iron deficiency anemia in pregnant rats and its mechanism[J]. Food and Fermentation Industries, 2026
, 52(9)
: 38
-43
.
DOI: 10.13995/j.cnki.11-1802/ts.044847
[1] 中华医学会血液学分会红细胞疾病(贫血)学组.铁缺乏症和缺铁性贫血诊治和预防的多学科专家共识(2022年版)[J].中华医学杂志, 2022, 102(41):3246-3256.
Chinese Society of Hematology, Erythrocytic Diseases (Anemia) Group.Multidisciplinary expert consensus on diagnosis, treatment and prevention of iron deficiency and iron deficiency anemia (2022 edition) [J].National Medical Journal of China, 2022, 102(41):3246-3256.
[2] KAMLUNGKUEA T, KAEWCHUNG C, SUBLON N, et al.Pregnancy outcomes among women with treated iron deficiency Anemia:A retrospective cohort study[J].Nutrients, 2025, 17(19):3168.
[3] IGBINOSA I, BERUBE C, LYELL D J.Iron deficiency anemia in pregnancy[J].Current Opinion in Obstetrics & Gynecology, 2022, 34(2):69-76.
[4] SHI W B, WANG Z X, LIU H B, et al.Study on the mechanism of Fufang E’jiao Jiang on precancerous lesions of gastric cancer based on network pharmacology and metabolomics[J].Journal of Ethnopharmacology, 2023, 304:116030.
[5] 郎海燕, 陈信义, 杨文华.缺铁性贫血中医药防治康复一体化专家共识[J].中华中医药杂志, 2018, 33(8):3487-3492.
LANG H Y, CHEN X Y, YANG W H.Expert consensus on the integration of TCM prevention and treatment for iron deficiency Anemia[J].China Journal of Traditional Chinese Medicine and Pharmacy, 2018, 33(8):3487-3492.
[6] 王莹雪, 樊雨梅, 廖峰, 等.阿胶活性肽的结构鉴定及活性筛选[J].食品科学, 2022, 43(10):207-213.
WANG Y X, FAN Y M, LIAO F, et al.Structure identification and activity screening of active peptides in Colla corii asini(Ejiao)[J].Food Science, 2022, 43(10):207-213.
[7] ITO T, NOCHIOKA K, NODA T, et al.New-onset anemia and its association with ventricular arrhythmias and sudden cardiac death in patients with heart failure with preserved ejection fraction[J].International Journal of Cardiology.Heart & Vasculature, 2025, 61:101812.
[8] GEORGIEFF M K.Iron deficiency in pregnancy[J].American Journal of Obstetrics and Gynecology, 2020, 223(4):516-524.
[9] GARCIA-CASAL M N, PASRICHA S R, MARTINEZ R X, et al.Serum or plasma ferritin concentration as an index of iron deficiency and overload[J].The Cochrane Database of Systematic Reviews, 2021, 5(5):CD011817.
[10] CHEN Y, WAN J, XIA H D, et al.Total iron binding capacity (TIBC) is a potential biomarker of left ventricular remodelling for patients with iron deficiency anaemia[J].BMC Cardiovascular Disorders, 2020, 20(1):4.
[11] ÅSBERG A, THORSTENSEN K, HOV G G, et al.Diagnosing empty iron stores in women:Unbound iron binding capacity (UIBC) versus soluble transferrin receptor (sTFR)[J].Scandinavian Journal of Clinical and Laboratory Investigation, 2021, 81(2):137-141.
[12] FROST J N, WIDEMAN S K, PRESTON A E, et al.Plasma iron controls neutrophil production and function[J].Science Advances, 2022, 8(40):eabq5384.
[13] PAGANI A, NAI A, CORNA G, et al.Low hepcidin accounts for the proinflammatory status associated with iron deficiency[J].Blood, 2011, 118(3):736-746.
[14] KANG M, KWON S, LEE W, et al.Soluble transferrin receptor can predict all-cause mortality regardless of anaemia and iron storage status[J].Scientific Reports, 2022, 12:11911.
[15] SATO K, INOUE S, IGARASHI A, et al.Effect of iron deficiency on a murine model of smoke-induced emphysema[J].American Journal of Respiratory Cell and Molecular Biology, 2020, 62(5):588-597.
[16] WANG J X, SUN Q H, WANG G W, et al.The effects of blunt snout bream (Megalobrama amblycephala) IL-6 trans-signaling on immunity and iron metabolism via JAK/STAT3 pathway[J].Developmental and Comparative Immunology, 2022, 131:104372.
[17] RAUNDHAL M, GHOSH S, MYERS S A, et al.Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias[J].Nature Immunology, 2021, 22(4):520-529.
[18] RU Y, PRESSMAN E K, COOPER E M, et al.Iron deficiency and anemia are prevalent in women with multiple gestations[J].The American Journal of Clinical Nutrition, 2016, 104(4):1052-1060.
[19] 尹晓晨, 龙晓蕾, 李理, 等.某复方软胶囊对缺铁性贫血改善效果[J].中国公共卫生, 2019, 35(11):1520-1523.
YIN X C, LONG X L, LI L, et al.Improvement effect of a compound soft capsule on iron deficiency anemia in community patients[J].Chinese Journal of Public Health, 2019, 35(11):1520-1523.
[20] 李勇. 肽临床营养学 [M].北京:北京大学医学出版社, 2012.
LI Y.Peptide Clinical Nutrition [M].Beijing:Peking University Medical Press, 2012.
[21] GAMBLING L, CHARANIA Z, HANNAH L, et al.Effect of iron deficiency on placental cytokine expression and fetal growth in the pregnant rat[J].Biology of Reproduction, 2002, 66(2):516-523.
