Food and Fermentation Industries >

2026 , Vol. 52 >Issue 9: 118 - 125

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043873

Isolation, identification, and inactivation kinetics of radiation-resistant bacteria in pepper

  • GU Xiaoyu ,
  • WANG Xian ,
  • YAN Huili ,
  • ZHANG Dong ,
  • CHEN Sisi ,
  • ZHANG Dianhe ,
  • WANG Rui ,
  • XIANG Qisen
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  • 1(College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China)
    2(Isotope Institute Co. Ltd., Henan Academy of Sciences, Zhengzhou 450015, China)
    3(Henan Dongfang Yichu Food Co.Ltd., Changge 461000, China)

Received date: 2025-07-11

  Revised date: 2025-09-04

  Online published: 2026-06-03

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Abstract

This work aimed to explore the radiation-resistant bacteria in pepper and clarify their resistance patterns.Pepper samples irradiated with a high-energy electron beam at a dose of 12 kGy were utilized as raw materials for the isolation and purification of bacteria.Radiation-resistant bacteria were identified through the physiological-biochemical characterization, Gram staining, and 16S rDNA sequencing.Meanwhile, the radiation resistance of the isolates was further evaluated.The inactivation kinetics of radiation-resistant bacteria after exposure to high-energy electron beam was fitted by the linear, Weibull, and Log-Logistic kinetic models.Three bacteria were isolated from the irradiated pepper, all of which were Gram-positive and spore-forming bacteria.The isolates were identified as Bacillus safensis, Bacillus velezensis, and Priestia megaterium, respectively.All three isolates exhibited resistance to ampicillin and penicillin.Compared to the linear and Log-Logistic models, the Weibull model accurately described the inactivation kinetics of these isolates after exposure to high-energy electron beam, with the highest R2 value (closest to 1) and the lowest root mean square error.This study elucidates the radiation resistance patterns of radiation-resistant bacteria, providing a theoretical foundation for controlling the radiation-resistant microorganisms in spices.

Key words： pepper; radiation-resistant bacteria; isolation and identification; 16S rDNA; kinetic model

Cite this article

GU Xiaoyu , WANG Xian , YAN Huili , ZHANG Dong , CHEN Sisi , ZHANG Dianhe , WANG Rui , XIANG Qisen . Isolation, identification, and inactivation kinetics of radiation-resistant bacteria in pepper[J]. Food and Fermentation Industries, 2026 , 52(9) : 118 -125 . DOI: 10.13995/j.cnki.11-1802/ts.043873

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