Food and Fermentation Industries >

2026 , Vol. 52 >Issue 9: 205 - 212

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044009

Screening of the suitable resin for separation and purification of sesame antioxidant peptides

  • LU Xin ,
  • ZHANG Lixia ,
  • SUN Xiaojing ,
  • SUN Qiang ,
  • SONG Guohui ,
  • HUANG Jinian
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  • 1(Center of Agricultural Products Processing of Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
    2(Henan Engineering Research Centre of Bioactive Substances in Agricultural Products, Zhengzhou 450002, China)
    3(Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

Received date: 2025-07-23

  Revised date: 2025-09-03

  Online published: 2026-06-03

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Abstract

To identify the appropriate resin for the separation and purification of the sesame antioxidant peptides, the performance of 20 resins belonging to 6 types on the static adsorption of sesame protein hydrolysate were investigated with peptide recovery, impurity residue and antioxidant activity as evaluation indexes, the molecular weight distribution and composition of absorbed and unabsorbed peptides originated from macroporous resin D3520 were analyzed, finally, the difference on structural characteristics between absorbed and unabsorbed peptides was explored.Results showed that the type and structure of resin significantly affected the behavior of isolation and purification of sesame antioxidant peptides by resins, such as ion exchange resins were suitable for removing impurities (amino acid, protein, salt, pigment) in enzymatic hydrolysate, while the nonpolar macroporous resin was good at enriching the sesame antioxidant peptide.According to the performance on antioxidant activity and recovery of peptide, impurity removal, D3520 was suitable to separate and purify the sesame antioxidant peptide.Compared with the unabsorbed peptides after treated by D3520, the molecular weight distribution of absorbed peptides was concentrated to the low molecular weight, the kinds of absorbed peptides were 1.6 times more than unabsorbed peptides, the absorbed peptides showed higher hydrophobicity, lower steric hindrance, and higher pI.These findings provide the preliminary basis for optimizing the separation and purification of sesame antioxidant peptide or other peptides by resin, and also provide the date reference for exploring the adsorption mechanism of resin to peptides.

Key words： macroporous resin; ion exchange resin; antioxidant activity; impurity removal; peptide composition

Cite this article

LU Xin , ZHANG Lixia , SUN Xiaojing , SUN Qiang , SONG Guohui , HUANG Jinian . Screening of the suitable resin for separation and purification of sesame antioxidant peptides[J]. Food and Fermentation Industries, 2026 , 52(9) : 205 -212 . DOI: 10.13995/j.cnki.11-1802/ts.044009

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