Food and Fermentation Industries >

2026 , Vol. 52 >Issue 10: 364 - 372

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044195

High-throughput screening and rapid quantification of 66 additives in alcoholic beverages by ultra performance liquid chromatography-tandem mass spectrometry

  • DU Juan ,
  • ZHOU Haiyan ,
  • LU Qian ,
  • HUANG Lijuan ,
  • WEI Yu ,
  • HE Yang ,
  • DU Gang ,
  • LI Daoxia
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  • 1(Luzhou Laojiao Co., Ltd.(National Engineering Research Center of Solid-State Brewing), Luzhou 646000, China)
    2(Sichuan Provincial Institute of Food Inspection and Research, Chengdu 610097, China)
    3(Key Laboratory of Baijiu Supervising Technology for State Market Regulation, Chengdu 610097, China)

Received date: 2025-08-11

  Revised date: 2025-11-01

  Online published: 2026-06-15

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Abstract

To achieve the simultaneous determination of 66 additives in alcoholic beverages, this study established a high-throughput analytical method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Alcoholic samples were processed using nitrogen evaporation.Methanol-acetonitrile (1∶1, V/V) and 5 mmol/L ammonium acetate aqueous solution were used as the mobile phase.Electrospray ionization (ESI) in both positive and negative modes was used in combination with dynamic multiple reaction monitoring (dMRM).Quantification was performed using matrix-matched external standard calibration curves.The results showed that all 66 additives exhibited good linearity within the specified concentration ranges (r>0.999).The average recovery ranged from 62.6% to 117.2%, with precision between 0.2% and 9.5%.Limits of detection (LOD) were 0.02-0.1 mg/L, and limits of quantification (LOQ) were 0.04-0.2 mg/L.Analysis of actual samples revealed that among 283 batches of Baijiu(Chinese distilled liquor), 8 sweeteners and 2 preservatives were detected.In 63.3% of bulk Baijiu samples, two or more sweeteners were found.In 16 batches of blended liquor, the types of additives detected did not match the label declarations.This method offers advantages such as high throughput, good reproducibility, high sensitivity, and rapid analysis.Compared to existing methods, this approach significantly improves detection efficiency and coverage for additive monitoring in alcoholic beverages.It meets the requirements for the fast determination of 66 additives in alcoholic beverages and provides reliable technical support for quality control and additive risk monitoring in alcoholic food products.

Key words： additives; sweeteners; preservatives; sugar alcohols; bulk Baijiu; ultra-high-performance liquid chromatography-tandem mass spectrometry; high-throughput

Cite this article

DU Juan , ZHOU Haiyan , LU Qian , HUANG Lijuan , WEI Yu , HE Yang , DU Gang , LI Daoxia . High-throughput screening and rapid quantification of 66 additives in alcoholic beverages by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2026 , 52(10) : 364 -372 . DOI: 10.13995/j.cnki.11-1802/ts.044195

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