To explore natural and novel functional foods for the prevention and treatment of chronic alcoholic liver injury (CALI), three novel active peptides complexes were developed through rational formulation.Their regulatory effects on liver protection, and inflammation in CALI mice were evaluated, and the underlying mechanisms involving oxidative stress, lipid metabolism, and gut microbiota modulation were investigated.Results showed that all three peptide complexes, particularly the concentrated formulation, effectively alleviated alcohol-induced behavioral changes in CALI mice.They significantly enhanced hepatic alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities, reduced liver index, and lowered serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), thereby improving hepatic histopathological damage.Moreover, these complexes downregulated hepatic TNF-α and IL-6 levels, while increasing superoxide dismutase, catalase, and glutathione contents, and reducing malondialdehyde levels.Additionally, they improved lipid profiles, including total cholesterol and triglycerides, and enhanced gut microbiota diversity and abundance.These findings provide a scientific basis for the development of novel functional foods with hepatoprotective and alcohol-metabolism-enhancing properties.
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