Food and Fermentation Industries >

2026 , Vol. 52 >Issue 15: 29 - 37

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045998

Antibacterial properties and mining of bacteriocin gene clusters of Lactiplantibacillus plantarum F44

  • BAI Yue ,
  • SUN Zhilan ,
  • WANG Kun ,
  • LIU Fang
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  • 1(College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
    2(Institute of Agricultural Product Processing,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

Received date: 2026-01-26

  Revised date: 2026-03-27

  Online published: 2026-08-20

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Abstract

This study aimed to screen lactic acid bacteria that have an inhibitory effect on the dominant spoilage bacterium of chilled chicken, Pseudomonas lundensis, analyze their antibacterial characteristics, and mine potential bacteriocin gene clusters based on whole-genome sequences, ultimately evaluating their effectiveness in preserving chilled chicken.The results indicated that the selected Lactiplantibacillus plantarum F44 had a significant inhibitory effect on P.lundensis, with a minimum inhibitory concentration value of 312 μg/mL.The crude bacteriocin extract was sensitive to proteases, remained highly active against bacteria at 40-121 ℃ and pH range of 2.0-10.0, and exhibited antibacterial activity against common Gram-positive and Gram-negative bacteria.Whole-genome analysis showed that strain L.plantarum F44 had a genome length of 3 746 814 bp, a GC content of 41.33%, and contained five bacteriocin gene clusters.After treatment of chilled chicken with F44 bacteriocin (2MIC), the total bacterial count decreased by 2.6 lg CFU/g and the thiobarbituric acid reactive substances content decreased by 0.35 mg/kg after 12 days of refrigeration, extending the shelf life by 3 days.In summary, L.plantarum F44 and the bacteriocins it produces have good antibacterial properties and preservation potential, providing new microbial resources and a theoretical basis for the development of natural preservatives, and have promising application prospects.

Key words： Lactiplantibacillus plantarum; whole genome sequencing; Pseudomonas lundensis; bacteriocin

Cite this article

BAI Yue , SUN Zhilan , WANG Kun , LIU Fang . Antibacterial properties and mining of bacteriocin gene clusters of Lactiplantibacillus plantarum F44[J]. Food and Fermentation Industries, 2026 , 52(15) : 29 -37 . DOI: 10.13995/j.cnki.11-1802/ts.045998

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