Food and Fermentation Industries >

2026 , Vol. 52 >Issue 15: 418 - 428

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044632

Application prospects of nanobodies in rapid detection and neutralization therapy of foodborne

  • YANG Chunxu ,
  • ZHU Lei ,
  • SU Na ,
  • JI Rimutu ,
  • TUYATSETSEG Jambal ,
  • YI Rule ,
  • LIANG Jianzhong ,
  • DA Lizhao ,
  • YI Li
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  • 1(China-Mongolia Biomacromolecule Application “Belt and Road” Joint Laboratory, InnerMongolia Agricultural University, Hohhot 010018, China)
    2(College of Food Science and Engineering, InnerMongolia Agricultural University, Hohhot 010018, China)
    3(Inner Mongolia China-Kazakhstan CamelResearch Institute, Alxa 737300, China)
    4(Inner Mongolia Guotuo Testing Technology Co. Ltd., Alxa 750300, China)
    5(Agricultural Technology Extension Center of Alxa Right Banner, Alxa 750300, China)
    6(Alashan Right Banner Camel Industry Development Center, Hohhot 737300, China)
    7(Mongolian University of Science and Technology, Ulaanbaatar 999097, Mongolia)

Received date: 2025-10-29

  Revised date: 2025-12-19

  Online published: 2026-08-20

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Abstract

Foodborne pathogens represent a significant threat to global food safety and public health.The escalating issue of antibiotic resistance has heightened the urgency for developing innovative detection and treatment strategies.Nanobodie single-domain antibody fragments derived from camelid heavy-chain antibodies, offer several advantages, including small molecular size, high stability, ease of modification, and cost-effective production.They have emerged as powerful tools for overcoming the limitations of traditional immunological methods.This review provides an overview of the latest applications of nanobodies in the detection and treatment of foodborne pathogens.In the realm of detection, emphasis is placed on immunological sensors based on nanobodies (including ELISA, electrochemical sensors, and lateral flow test strips),highlighting their advantages in enhancing sensitivity, reducing detection time, and enabling point-of-care testing.Regarding therapeutic applications, this study examines the potential of nanobodies in antibacterial treatment through multiple mechanisms, including bacterial adhesion inhibition and toxin neutralization.Lastly, this review discusses the challenges and future directions for the use of nanobodies in this field, with the aim of providing a theoretical foundation for their advancement and translation into food safety and clinical anti-infective applications.

Key words： nanobodies; foodborne pathogens; immunoassays; neutralization therapy; food safety; public health

Cite this article

YANG Chunxu , ZHU Lei , SU Na , JI Rimutu , TUYATSETSEG Jambal , YI Rule , LIANG Jianzhong , DA Lizhao , YI Li . Application prospects of nanobodies in rapid detection and neutralization therapy of foodborne[J]. Food and Fermentation Industries, 2026 , 52(15) : 418 -428 . DOI: 10.13995/j.cnki.11-1802/ts.044632

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