Food and Fermentation Industries >

2026 , Vol. 52 >Issue 14: 195 - 202

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044199

Impact of multi-walled carbon nanotubes on biogas production performance in electrocatalytic anaerobic digestion

  • ZENG Jiakai ,
  • ZHOU Dan ,
  • JIANG Dongyun ,
  • CHEN Rongbin
Expand
  • (School of Life & Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, China)

Received date: 2025-08-11

  Revised date: 2025-11-24

  Online published: 2026-08-21

Fold

Abstract

Multi-walled carbon nanotubes (MWCNTs) were employed to optimize methanogenic performance in an electrocatalytic anaerobic digestion (EAD) system.Scanning electron microscopy and high-throughput sequencing were applied to investigate the electroactive methanogens' response to MWCNTs and MWCNT-mediated electron transfer mechanisms.When acetate was supplied as the sole substrate, the relative abundance of the normally minor methanogen Methanosarcina recovered from 0.10% to 6.79% in the MWCNTs-amended EAD reactors.SEM imaging further revealed distinct “microbe-carbon nanotube” composite structure between MWCNTs and methanogenic cells, demonstrating a newly established route for extracellular electron transfer within the EAD process.

Key words： electrocatalytic anaerobic digestion; multi-walled carbon nanotubes; direct interspecies electron transfer; Methanosarcina; MWCNTs-mediated

Cite this article

ZENG Jiakai , ZHOU Dan , JIANG Dongyun , CHEN Rongbin . Impact of multi-walled carbon nanotubes on biogas production performance in electrocatalytic anaerobic digestion[J]. Food and Fermentation Industries, 2026 , 52(14) : 195 -202 . DOI: 10.13995/j.cnki.11-1802/ts.044199

References

[1] PARRA R, CHICAIZA-ORTIZ C, HERRERA-FEIJOO R J, et al.Advancements of biohydrogen production based on anaerobic digestion:Technologies, substrates, and future prospects[J].Sci, 2025, 7(2):52.
[2] 梁家伟, 周亶, 蒋东云, 等.生物电化学系统强化厌氧消化的机理和底物效应[J].中国沼气, 2023, 41(6):26-33.
LIANG J W, ZHOU D, JIANG D Y, et al.Mechanism and substrate effect of bioelectrochemical system enhanced anaerobic digestion[J].China Biogas, 2023, 41(6):26-33.
[3] AN Z K, SONG Y C, KIM K T, et al.The bioaugmentation of electroactive microorganisms enhances anaerobic digestion[J].Fermentation, 2023, 9(11):988.
[4] AO T J, ZHAO X Q, MEHMOOD M A, et al.A double-chamber microbial electrolysis cell improved the anaerobic digestion efficiency and elucidated the underlying bio-electrochemical mechanism[J].Chemical Engineering Journal, 2023, 471:144228.
[5] 康厚智, 周亶, 刘志男, 等.电催化厌氧消化提高沼气中甲烷含量[J].食品与发酵工业, 2025,51(15):152-159.
KANG H Z, ZHOU D, LIU Z N, et al.Electrocatalytic anaerobic digestion to increase methane content in biogas[J].Food and Fermentation Industries, 2025, 51(15):152-159.
[6] GOMEZ VIDALES A, OMANOVIC S, TARTAKOVSKY B.Combined energy storage and methane bioelectrosynthesis from carbon dioxide in a microbial electrosynthesis system[J].Bioresource Technology Reports, 2019, 8:100302.
[7] VASILIADOU I A, KALOGIANNIS A, SPYRIDONIDIS A, et al.Effect of applied potential on the performance of an electroactive methanogenic biocathode used for bioelectrochemical CO2 reduction to CH4[J].Journal of Chemical Technology & Biotechnology, 2022, 97(3):643-652.
[8] LOVLEY D R.Syntrophy goes electric:Direct interspecies electron transfer[J].Annual Review of Microbiology, 2017, 71:643-664.
[9] AKRAM J, SONG C, EL MASHAD H M, et al.Advances in microbial community, mechanisms and stimulation effects of direct interspecies electron transfer in anaerobic digestion[J].Biotechnology Advances, 2024, 76:108398.
[10] 吴彤彤, 孙辰, 曹卫星. 纳米Fe2O3及接种比对餐厨垃圾厌氧消化性能与微生物群落结构的影响机制[J]. 浙江理工大学学报(自然科学), 2026, 51(1): 66-74.
WU T T, SUN C, CAO W X. The influence mechanism of nano-Fe2O3 and inoculum to substrate ratios on the anaerobic digestion performance and microbial community structure of food waste[J]. Journal of Zhejiang Sci-Tech University (Natural Sciences), 2026, 51(1): 66-74.
[11] WEI S J, GUO M, GUO M X, et al.Sludge-based iron-carbon composite mediators enhanced simultaneous utilization of anaerobic sludge and biogas slurry in anaerobic digestion coupled with microbial electrolysis cell[J].Bioresource Technology, 2025, 438:133247.
[12] CAVALEIRO A, SALVADOR A, MARTINS G, et al.Multi-walled carbon nanotubes enhance methanogenesis from diverse organic compounds in anaerobic sludge and river sediments[J].Applied Sciences, 2020, 10(22):8184.
[13] ZHENG S L, LI Z, ZHANG P, et al.Multi-walled carbon nanotubes accelerate interspecies electron transfer between Geobacter cocultures[J].Bioelectrochemistry, 2020, 131:107346.
[14] 葛振朋, 李晓毅.管径和氮掺杂对纳米管中氧气分子传输的影响[J].中国科学院大学学报, 2014, 31(1):135-138.
GE Z P, LI X Y.Effects of diameter and N-doping on oxygen molecule transportation through a nanotube[J].Journal of University of Chinese Academy of Sciences, 2014, 31(1):135-138.
[15] LV Y J, CHEN M.Molecular transport through finite-length carbon nanotubes[J].Acta Physico-Chimica Sinica, 2012, 28(5):1070-1076.
[16] 郑锐, 蒋东云, 农以宁, 等.Logistic函数在连续投料厌氧消化过程分析中的应用[J].中国沼气, 2019, 37(6):19-24.
ZHENG R, JIANG D Y, NONG Y N, et al.Application of logistic function in analysis of continuous feeding anaerobic digestion process[J].China Biogas, 2019, 37(6):19-24.
[17] CONKLIN A, STENSEL H D, FERGUSON J.Growth kinetics and competition between Methanosarcina and Methanosaeta in mesophilic anaerobic digestion[J].Water Environment Research, 2006, 78(5):486-496.
[18] WANG Z X, WANG T F, SI B C, et al.Accelerating anaerobic digestion for methane production:Potential role of direct interspecies electron transfer[J].Renewable and Sustainable Energy Reviews, 2021, 145:111069.
[19] YEE M O, SNOEYENBOS-WEST O L, THAMDRUP B, et al.Extracellular electron uptake by two Methanosarcina species[J].Frontiers in Energy Research, 2019, 7:29.
[20] JUNG H, YU H, LEE C.Direct interspecies electron transfer enables anaerobic oxidation of sulfide to elemental sulfur coupled with CO2-reducing methanogenesis[J].iScience, 2023, 26(9):107504.
[21] LIU C Q, SUN D Z, ZHAO Z Q, et al.Methanothrix enhances biogas upgrading in microbial electrolysis cell via direct electron transfer[J].Bioresource Technology, 2019, 291:121877.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.