Transcriptional response of alcohol-resistant Hanseniaspora uvarum (GJ3) to ethanol stress

  • SU Jie ,
  • LI Yunlong ,
  • HU Haixia ,
  • SHI Xiaoxia ,
  • WANG Yanrong
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  • (College of Vocational Technology, Inner Mongolia Agricultural University, Baotou 014109, China)

Received date: 2025-08-28

  Revised date: 2025-11-28

  Online published: 2026-08-21

Abstract

One strain of Hanseniaspora uvarum,designated GJ3, was isolated from naturally fermented grape mash in the Wuhai region in our laboratory.This strain exhibits strong ethanol tolerance.To elucidate its ethanol stress response mechanism, the effects of varying ethanol concentrations on its growth were investigated, and transcriptomic analysis was performed after exposure to 6% ethanol for 2 hours.The results indicated that ethanol inhibits the growth of H.uvarum, with higher ethanol concentrations leading to more pronounced inhibitory effects.Transcriptome sequencing revealed a total of 1 090 differentially expressed genes under ethanol stress, which were predominantly enriched in pathways related to energy metabolism, carbohydrate and its derivative metabolism, cell signal transduction, genetic information processing, transport, and catabolic processes.Ethanol stress may lead to vacuolar acidification and altered membrane fluidity, impair proteolytic activity, and reduce ATP synthesis capacity.The strain may counteract membrane damage and oxidative stress by reducing energy consumption, suppressing anabolic processes, minimizing the accumulation of toxic metabolites, and modulating the expression of cell wall-associated genes, RNA degradation pathways, and the HOG-MAPK signaling pathway.This study provided a theoretical foundation for further investigation into the mechanisms underlying ethanol stress in H.uvarum and for the development of genetically engineered strains with enhanced ethanol tolerance.

Cite this article

SU Jie , LI Yunlong , HU Haixia , SHI Xiaoxia , WANG Yanrong . Transcriptional response of alcohol-resistant Hanseniaspora uvarum (GJ3) to ethanol stress[J]. Food and Fermentation Industries, 2026 , 52(14) : 246 -254 . DOI: 10.13995/j.cnki.11-1802/ts.044353

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