Food and Fermentation Industries >

2026 , Vol. 52 >Issue 14: 343 - 350

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044605

Simultaneous determination of 22 sugar alcohols and sugar in fermentation broth by ion chromatography

  • JIANG Lili ,
  • HAN Mei ,
  • LU Wanpin ,
  • CHEN Jiugeng
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  • 1(Shanghai Jjiaotong University, Instrumental Analysis Center, Shanghai 200240, China)
    2(Shanghai Business School, Food Quality and Safety, Shanghai 200235, China)

Received date: 2025-09-22

  Revised date: 2025-12-22

  Online published: 2026-08-21

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Abstract

This paper has established an ion chromatography (IC)-pulsed amperometric detector (PAD) method with high sensitivity for simultaneously determining various sugars such as monosaccharides, disaccharides, sugar alcohols and uronic acid, which can be used to characterize the utilization sequence of different carbon sources by microorganisms in a complex combination of carbon sources.Through the study of factors such as column type, eluent concentration, column temperature and flow rate, an analytical method for the simultaneous determination of the contents of 22 sugar components within 55 minutes was established.in fermentation.By investigating the key parameters such as elution concentration, column temperature, and flow rate, we established an analytical method capable of quantifying all 22 components within 55 minutes.Experimental results demonstrated that at a flow rate of 0.45 mL/min, using gradient elution with sodium hydroxide and sodium acetate, and maintaining a column temperature of 30 ℃, all 22 components exhibited good linearity (r2>0.995) within the concentration range of 0.1 to 10 mg/L.The limits of detection (LODs) for these components ranged from 0.005 to 0.098 mg/L.At three concentration levels of 0.5 mg/L, 2.0 mg/L, and 4.0 mg/L, the average recovery rates for the 22 components ranged from 77% to 110%.This method demonstrates high efficiency, sensitivity, and accuracy and has been applied to the investigate the preference of Streptococcus thermophilus for different types of carbon sources.

Key words： ion chromatography; zymotic fluid; sugar alcohols; monosaccharide; oligosaccharide; content

Cite this article

JIANG Lili , HAN Mei , LU Wanpin , CHEN Jiugeng . Simultaneous determination of 22 sugar alcohols and sugar in fermentation broth by ion chromatography[J]. Food and Fermentation Industries, 2026 , 52(14) : 343 -350 . DOI: 10.13995/j.cnki.11-1802/ts.044605

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