Food and Fermentation Industries >

2026 , Vol. 52 >Issue 16: 17 - 24

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.046685

Recombinant expression and enzymatic properties of a glucose- and xylose-stimulated thermophilic β-glucosidase from Thermomicrobium roseum

  • LIU Yang ,
  • HUANG Jie ,
  • ZHANG Tao ,
  • DOU Kangjie ,
  • XU Youshan ,
  • ZHAO Weiping
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  • 1(School of Biological and Food Engineering, Chuzhou University, Chuzhou 239000, China)
    2(School of Life Sciences and Medical Engineering, Anhui University, Hefei 230601, China)
    3(Tianchang Shengfeng Eco-Agricultural Technology Co.Ltd., Chuzhou 239000, China)

Received date: 2026-03-26

  Revised date: 2026-04-08

  Online published: 2026-09-10

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Abstract

To address the low catalytic activity, poor thermostability, and feedback inhibition by hydrolysis products commonly observed in β-glucosidase applications, this study identified a novel β-glucosidase Bgl95 from Thermomicrobium roseum and comprehensively evaluated its enzymatic properties and application potential.Bgl95 exhibited a molecular mass of 52 kDa, with optimal activity at 90 ℃ and pH 7.5.Using pNPG as substrate, the specific activity reached (310.5±4.1) U/mg, with a Km of (6.1±0.5) mmol/L and Vmax of (163.9±3.6) μmol/(min·mg).Bgl95 specifically hydrolyzed β-1,4-glycosidic bonds and natural substrates, including cellobiose and lactose.Enzyme activity increased significantly in the presence of 5 mmol/L Fe2+, Mg2+ and 1% Tween 80, whereas 5 mmol/L Co2+, Cu2+ and 1% SDS showed obvious inhibitory effects.Notably, Bgl95 represented a rare glucose- and xylose- stimulated β-glucosidase and 0.8 mol/L glucose and 1.8 mol/L xylose enhanced enzyme activity by 3.55-fold and 4-fold, respectively.Even in the presence of 4 mol/L glucose or xylose, Bgl95 maintained 51% and 139% of the enzyme activity.These findings provided theoretical support for the application of Bgl95 in cellulose degradation and food fermentation.

Key words： Thermomicrobium roseum; glucose- and xylose-stimulated; β-glucosidase; recombinant expression; enzymatic properties

Cite this article

LIU Yang , HUANG Jie , ZHANG Tao , DOU Kangjie , XU Youshan , ZHAO Weiping . Recombinant expression and enzymatic properties of a glucose- and xylose-stimulated thermophilic β-glucosidase from Thermomicrobium roseum[J]. Food and Fermentation Industries, 2026 , 52(16) : 17 -24 . DOI: 10.13995/j.cnki.11-1802/ts.046685

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