Food and Fermentation Industries >

2026 , Vol. 52 >Issue 16: 80 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044538

Modulation of gut microbiota and short-chain fatty acids by a specific synbiotic in mice with loperamide hydrochloride-induced constipation

  • LU Shaoshuai ,
  • MA Weiwei ,
  • ZHANG Ziwei ,
  • XU Ruixin ,
  • HUANG Lili
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  • 1(College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150006, China)
    2(Changchun Jinsai Pharmaceutical Co.Ltd., Changchun 130015, China)

Received date: 2025-09-18

  Revised date: 2026-01-06

  Online published: 2026-09-10

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Abstract

Previous studies confirmed the laxative effect of this synbiotic.Building on this, this study investigated its dose-dependent effects on gut microbiota and short-chain fatty acids (SCFAs) in constipated mice from a microecological perspective.A synbiotic containing Lactiplantibacillus plantarum CCFM8661, Bifidobacterium animalis subsp.lactis XLTG11, and xylooligosaccharides (XOS) was administered to ICR mice at low, medium, and high doses for 14 days.Constipation was then induced with loperamide hydrochloride.Fecal samples were analyzed via metagenomic sequencing and SCFA profiling.Synbiotic intervention dose-dependently modulated gut microbiota.All doses increased beneficial genera like Bifidobacterium and Faecalibaculum while reducing Butyricimonas.Medium-dose intervention best restored microbial diversity and structure, whereas high-dose significantly lowered ACE and observed species indices (P<0.05).SCFA profiles in low- and medium-dose groups resembled the blank group, but the high-dose group showed significant changes.This synbiotic alleviates constipation by remodeling gut microbiota and regulating SCFA metabolism.The medium dose (3×106 CFU+2 mg XOS) demonstrated optimal regulatory potential by balancing microbiota structure and metabolic function.

Key words： synbiotic; probiotics; constipation; gut microbiota; short-chain fatty acids (SCFAs)

Cite this article

LU Shaoshuai , MA Weiwei , ZHANG Ziwei , XU Ruixin , HUANG Lili . Modulation of gut microbiota and short-chain fatty acids by a specific synbiotic in mice with loperamide hydrochloride-induced constipation[J]. Food and Fermentation Industries, 2026 , 52(16) : 80 -88 . DOI: 10.13995/j.cnki.11-1802/ts.044538

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