Food and Fermentation Industries >

2026 , Vol. 52 >Issue 17: 272 - 281

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045120

Comparative metabolomic analysis of back and abdominal muscles in wild and cultured Larimichthys crocea

  • WEI Shuai ,
  • LIANG Shan ,
  • YANG Yuying ,
  • WANG Zefu ,
  • LIU Yang ,
  • YIN Yantao ,
  • XIAO Naiyong ,
  • LIU Shucheng
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2025-11-10

  Revised date: 2025-12-20

  Online published: 2026-09-21

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Abstract

Wild and cultured Larimichthys crocea differ in habitat, feeding patterns, and physiological activities, which may lead to variations in muscle quality and metabolic characteristics.In this study, back and abdominal muscles from wild and cultured L.crocea were analyzed using liquid chromatography-mass spectrometry-based non-targeted metabolomics to compare metabolic profiles and enriched pathways.The results showed abundant differential metabolites between two groups, with the largest number identified between the back muscle of wild fish and the abdominal muscle of cultured fish (188 metabolites).Principal component analysis and orthogonal partial least squares discriminant analysis effectively distinguished wild from cultured samples without model overfitting.Hierarchical clustering revealed that wild fish were enriched in cis-4-hydroxy-L-proline and phosphatidylcholines, whereas cultured fish contained higher levels of lipid derivatives and small molecules associated with feed additives or aquaculture environments.KEGG pathway analysis indicated that differential metabolites were mainly involved in amino acid metabolism, lipid metabolism, and energy metabolism.These findings suggest that the superior muscle quality of wild L.crocea is closely related to natural feeding and swimming activity, while adjusting feed composition and aquaculture conditions may help optimize metabolic pathways and improve the sensory and nutritional qualities of cultured fish.This study provides a molecular basis for quality evaluation and the optimization of farming strategies forL.crocea.

Key words： Larimichthys crocea; back and abdominal muscles; metabolomics; differential metabolites

Cite this article

WEI Shuai , LIANG Shan , YANG Yuying , WANG Zefu , LIU Yang , YIN Yantao , XIAO Naiyong , LIU Shucheng . Comparative metabolomic analysis of back and abdominal muscles in wild and cultured Larimichthys crocea[J]. Food and Fermentation Industries, 2026 , 52(17) : 272 -281 . DOI: 10.13995/j.cnki.11-1802/ts.045120

References

[1] Zheng J L, Wan F G, Chen Y L, et al.Comparative study on the morphological characteristics, nutritional quality, and tastes of large yellow croaker from five cage culture areas:Relay farming improved fish quality[J].Aquaculture, 2024, 590:741030.
[2] 邹国华, 宋炜, 谢正丽.大黄鱼深远海大型围栏养殖技术[J].中国水产, 2021(6):57-60.
Zou G H, Song W, Xie Z L.Advances in deep-sea large-scale enclosure farming techniques for Larimichthys croceas[J].China Fisheries, 2021(6):57-60.
[3] 李松, 郭全友, 李保国, 等.养殖模式和饵料对养殖大黄鱼体色、质构和营养成分评价的影响[J].食品与发酵工业, 2019, 45(21):118-125.
Li S, Guo Q Y, Li B G, et al.Evaluation of culture mode and feed on body color, texture and nutritional composition of cultured Pseudosciaena crocea[J].Food and Fermentation Industries, 2019, 45(21):118-125.
[4] 陈志, 李水根, 许丽双, 等.深远海养殖大黄鱼肌肉品质分析与比较[J].福建农业科技, 2024, 55(11):25-31.
Chen Z, Li S G, Xu L S, et al.Analysis and comparison of the muscle quality of Larimichthys croceas cultured in deep sea[J].Fujian Agricultural Science and Technology, 2024, 55(11):25-31.
[5] 石钰琢, 郭全友, 郑尧, 等.不同养殖模式及流通方式的大黄鱼品质评价和等级判定[J].食品科学, 2022, 43(13):184-191.
Shi Y Z, Guo Q Y, Zheng Y, et al.Quality evaluation and grading of pseudosciaena crocea cultured in different aquaculture modes and circulated under different conditions[J].Food Science, 2022, 43(13):184-191.
[6] 李国道, 徐昙烨, 朱丹妮, 等.代谢组学在水产品品质研究中的应用进展[J].广东海洋大学学报, 2023, 43(5):119-125.
Li G D, Xu T Y, Zhu D N, et al.Progress in application of metabolomics in the study of aquatic product quality[J].Journal of Guangdong Ocean University, 2023, 43(5):119-125.
[7] 高淑芳, 张金鹏, 施永海, 等.基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析[J].南方水产科学, 2022, 18(3):68-75.
Gao S F, Zhang J P, Shi Y H, et al.Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J].South China Fisheries Science, 2022, 18(3):68-75.
[8] Liu Z Y, Xiao N Y, Qin L, et al.Insights into volatile substance changes of tilapia fillets before and after steaming and boiling during refrigeration based on metabolomics[J].Food Chemistry, 2025, 493:145856.
[9] Cheng R, Ying Z Q, Yang Y, et al.Changes of intestinal microbiota and liver metabolomics in yellow catfish (Pelteobagrus fulvidraco) before and after rice flowering in rice-fish symbiosis farmed mode[J].Frontiers in Microbiology, 2025, 16:1617168.
[10] Dunn W B, Broadhurst D, Begley P, et al.Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry[J].Nature Protocols, 2011, 6(7):1060-1083.
[11] Zelena E, Dunn W B, Broadhurst D, et al.Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum[J].Analytical Chemistry, 2009, 81(4):1357-1364.
[12] Want E J, Masson P, Michopoulos F, et al.Global metabolic profiling of animal and human tissues via UPLC-MS[J].Nature Protocols, 2013, 8(1):17-32.
[13] Saccenti E, Hoefsloot H C J, Smilde A K, et al.Reflections on univariate and multivariate analysis of metabolomics data[J].Metabolomics, 2014, 10(3):361-374.
[14] Zheng J L, Chen Y L, Wan F G, et al.Comparative study on the quality of wild and ecologically farmed large yellow croaker through on-site synchronous sampling from the Nanji Archipelago in the East China Sea[J].Aquaculture, 2024, 591:741098.
[15] 杨航, 袁泉, 吕巍巍, 等.基于非靶向代谢组学分析野生和养殖黄鳝肌肉的代谢差异[J].上海农业学报, 2025, 41(5):16-25.
Yang H, Yuan Q, Lü W W, et al.Metabolic differences between wild and farmed Monopterus albus muscle analyzed by untargeted metabolomics[J].Acta Agriculturae Shanghai, 2025, 41(5):16-25.
[16] Chu Y M, Wang J F, Xie J.Foodomics in aquatic products quality assessment during storage:An advanced and reliable approach[J].Food Bioscience, 2024, 58:103734.
[17] Du H Y, Xiong S B, Lv H, et al.Comprehensive analysis of transcriptomics and metabolomics to understand the flesh quality regulation of crucian carp (Carassius auratus) treated with short term micro-flowing water system[J].Food Research International, 2021, 147:110519.
[18] Zhuang J C, Wang Y C, Shen W L, et al.Evaluating dynamic effects of dietary glycerol monolaurate on the productive performance and flesh quality of large yellow croaker (Larimichthys crocea)[J].Food Chemistry, 2022, 387:132833.
[19] Wang Y, Li W Q, Zhang C, et al.Metabolomic comparison of meat quality and metabolites of geese breast muscle at different ages[J].Food Chemistry:X, 2023, 19:100775.
[20] Cheng H, Mei J, Xie J.The odor deterioration of tilapia (Oreochromis mossambicus) fillets during cold storage revealed by LC-MS based metabolomics and HS-SPME-GC-MS analysis[J].Food Chemistry, 2023, 427:136699.
[21] Zheng Y, Wang X C.Adequate pre-freezing handling slows the quality deterioration of frozen obscure pufferfish:Revealed by untargeted metabolomics[J].Food Research International, 2023, 173:113423.
[22] Rao G D, Sui J K, Zhang J G.Metabolomics reveals significant variations in metabolites and correlations regarding the maturation of walnuts (Juglans regia L.)[J].Biology Open, 2016, 5(6):829-836.
[23] Enkerlin A, Hoffmann U A, Rapp J, et al.Deciphering the metabolic details of L-lysine toxicity in cyanobacteria[J].Plant Physiology,2026,200(4):kiag106.
[24] Qiu L L, Huang Q Q, Li W H, et al.Aging influences protein digestion, absorption and amino acid metabolism[J].Biogerontology, 2025, 26(4):146.
[25] Zeng L, Wang Y H, Ai C X, et al.Different cold tolerances among three strains of large yellow croaker:Related to antioxidant defense and energy metabolism[J].Fish Physiology and Biochemistry, 2023, 49(3):471-486.
[26] 周奎, 王润东, 宫梦学, 等.冷鲜波动温度对生食三文鱼肌肉代谢物的影响[J].食品科学, 2024, 45(22):229-238.
Zhou K, Wang R D, Gong M X, et al.Effect of temperature fluctuations during chilled storage on the metabolite composition of salmon consumed raw[J].Food Science, 2024, 45(22):229-238.
[27] Xu C C, Zang M W, Qiao X L, et al.Effects of ultrasound-assisted thawing on lamb meat quality and oxidative stability during refrigerated storage using non-targeted metabolomics[J].Ultrasonics Sonochemistry, 2022, 90:106211.
[28] Li Z D, Qi F Y, Li F.Integrating 16S sequencing and metabolomics study on anti-rheumatic mechanisms against collagen-induced arthritis of Wantong Jingu Tablet[J].Chinese Journal of Natural Medicines, 2022, 20(2):120-132.
[29] Bombardier E, Booth R K, Green H J, et al.Metabolic adaptations of oxidative muscle during spawning migration in the Atlantic salmon Salmo salar L[J].Fish Physiology and Biochemistry, 2010, 36(3):355-365.
[30] Joshi A, Basak T, Mondal B.Decoding elegant interplay among different stereo-electronic effects due to the ancient prolyl-4-hydroxylation stabilizing collagenous helicity[J].iScience, 2025, 28(5):112393.
[31] 米红波, 鹿悦, 陈敬鑫, 等.牛磺酸和谷氨酰胺对石斑鱼的应激缓解作用[J].水产科学, 2024, 43(6):865-874.
Mi H B, Lu Y, Chen J X, et al.Stress relieving effect of taurine and glutamine on grouper hybrid[J].Fisheries Science, 2024, 43(6):865-874.
[32] 刘伟, 蒋明, 吴凡, 等.饲料能量密度和投喂水平对吉富罗非鱼生长和健康的影响[J].水生生物学报, 2022, 46(1):58-68.
Liu W, Jiang M, Wu F, et al.Effects of dietary energy density and feed ration on growth and health in genetically improved farmed tilapia, Oreochromis niloticus[J].Acta Hydrobiologica Sinica, 2022, 46(1):58-68.
[33] 李媛洁, 陈作志, 张俊, 等.南海珊瑚礁海域蜂巢石斑鱼生物学特征研究[J].南方水产科学, 2021, 17(6):22-30.
Li Y J, Chen Z Z, Zhang J, et al.Biological characteristics of Epinephelus merra of coral reef waters in South China Sea[J].South China Fisheries Science, 2021, 17(6):22-30.
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