Fermentation was optimized for succinic acid production of recombinant Yarrowia lipolytica PGC11505 using 2.5-L bench-top fermenter.. The results showed that, the output of succinic acid was enhanced substantially after the optimization of speed of revolution, aeration rates, initial concentrations of glycerol and peptone, pH value of the fermenter. For detail, production of succinic acid was doubled under the speed of 600 r/min versus 400 r/min. Aeration rate of 1.0 vvm was appropriate when compared with 0.5 vvm and 2.0 vvm. Oxygen was deficient if aeration rate was low and it would waste power and energy when aeration rate was high. PGC11505 grew well and fermentation was done totally when the initial glycerol concentration was 100 g/L, the output of succinic acid reached 21.0 g/L. It was optimal for succinic production with 10.0 g/L peptone. PGC11505 performed the best when the pH value was kept natural as compared with pH value of 4.0, 5.0 and 6.0, which achieved 26.3 g/L succinic acid and a yield of 0.27 g/g. Finally, fed-batch fermentation was taken under natural pH value for 138 hours. A total of 192.5 g/L glycerol was consumed and 46.9 g/L succinic acid was produced with a yield of 0.24 g/g and productivity of 0.34 g/g/h. After optimization, the output of succinic acid was improved by 4.7.