Screening of extracellular protease-producing haloarchaea from Chaidamin salt lake and its enzyme properties
(School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
Haloarchaeal strains isolated from Chaidamin Salt Lake, Inner Mongolia were screened for high protease activity. Their enzyme properties were studied. The results showed that CDM10 and CDM12 displayed the highest protease activity, and represented novel taxa. The optimal protease activity of CDM10 occurred at 70-75 °C, pH 8.0-9.0, and 1.5 mol/L NaCl. The CDM12 protease exhibited maximal activity at 60-75 °C, pH 7.0-9.0, and 1.5-2.5 mol/L NaCl. The CDM10 and CDM12 proteases were highly stable over wide ranges of temperatures and NaCl concentrations. The inhibitor experiment suggested that the CDM10 and CDM12 proteases belonged to serine protease family, and the cysteine residues and metal ions were important for the enzyme activities.
Key words： extracellular protease; halophilic archaea; enzyme properties; salt lake
HOU Jing et al.
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Screening of extracellular protease-producing haloarchaea
from Chaidamin salt lake and its enzyme properties[J]. Food and Fermentation Industries, 0
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