Starter agents used in yogurt production are mostly good fermentation lactic acid bacteria, but not probiotics strains. It is necessary condition of lactic acid bacteria as probiotics that strains must go through gastrointestinal environment, then adhere and colonize in the intestinal tract. In this paper, the highly tolerant lactic acid bacteria strains isolated from the ethnic traditional food were screened by detecting the survival rate of strains in the artificial gastrointestinal solution, Then the potential application on yogurt of these strains were also comprehensively evaluated by the sensory evaluation and the texture analyzer. The results showed that survival rate of 7 strains were more than 50% in the 4 kinds of artificial gastrointestinal solution among 58 strains of lactic acid bacteria. The score of sensory evaluation of 5 strains are higher. The "Xin E6" demonstrated outstanding on yoghourt texture rupture force, the maximum e adhesion force, hardness, cohesiveness, elasticity, tackiness, chewiness and viscosity, holding water capacity. The "Xin E6" is Enterococcus durans by molecular biology methods. “Xin E6" has potential application in the development of probiotics starter agent.