CRISPR/Cas9 knockout strategy for essential genes in Escherichia coli and efficient plasmid replacement methods

LIU Jinhui, HUANG Jianfeng, YOU Xiaoyan, ZHANG Yanfei

Food and Fermentation Industries ›› 2023, Vol. 49 ›› Issue (18) : 17-23.

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Food and Fermentation Industries ›› 2023, Vol. 49 ›› Issue (18) : 17-23. DOI: 10.13995/j.cnki.11-1802/ts.035910

CRISPR/Cas9 knockout strategy for essential genes in Escherichia coli and efficient plasmid replacement methods

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{{article.zuoZheEn_L}}. {{article.title_en}}[J]. {{journal.qiKanMingCheng_EN}}, 2023, 49(18): 17-23 https://doi.org/10.13995/j.cnki.11-1802/ts.035910

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