Metagenomic analysis reveals dietary structure-dependent gut microbiota characteristics in non-alcoholic fatty liver disease patients

XIAO Rui; GUO Jianfeng; WANG Linlin; TIAN Peijun; JIN Xing; ZHAO Jianxin; CHEN Wei; WANG Gang

doi:10.13995/j.cnki.11-1802/ts.038975

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Food and Fermentation Industries ›› 2025, Vol. 51 ›› Issue (4) : 123-130. DOI: 10.13995/j.cnki.11-1802/ts.038975

Metagenomic analysis reveals dietary structure-dependent gut microbiota characteristics in non-alcoholic fatty liver disease patients

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Abstract

Non-alcoholic fatty liver disease (NAFLD) stands as a prevalent metabolic disorder globally, with its pathogenesis intricately linked to the gut microbiome.Given the diverse outcomes of numerous studies on gut microbial markers in NAFLD, the impact of dietary variations warrants examination.This investigation incorporated thirty NAFLD patients with distinct dietary patterns, categorizing them into two groups:fifteen individuals consuming a high-fat diet and another fifteen on a high-carbohydrate regimen.Employing metagenomic sequencing, this study analyzed the gut microbiota composition of these cohorts.Notably, significant discrepancies were observed in the microbial communities between the two dietary models.Specifically, individuals with prolonged high carbohydrate consumption predominantly harbored Clostridium species, whereas those with a sustained high-fat intake were characterized by an abundance of Faecalibacterium prausnitzii. From a functional standpoint, the microbiota of high-fat consumers showed enrichment in the bile acid metabolic pathway, whereas those on high-carbohydrate diets demonstrated a predominance in the palmitic acid synthesis pathway.These findings underscore the critical influence of dietary structure on gut microbiota composition in NAFLD patients and highlight the potential for targeted dietary interventions to modulate microbial pathways relevant to NAFLD pathogenesis.The insights gleaned from this study pave the way for tailored animal models of NAFLD and advocate for scientifically grounded dietary recommendations for individuals with this condition.

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metagenomics / non-alcoholic fatty liver disease / gut microbiota / dietary structure

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XIAO Rui, GUO Jianfeng, WANG Linlin, TIAN Peijun, JIN Xing, ZHAO Jianxin, CHEN Wei, WANG Gang. Metagenomic analysis reveals dietary structure-dependent gut microbiota characteristics in non-alcoholic fatty liver disease patients[J]. Food and Fermentation Industries, 2025, 51(4): 123-130 https://doi.org/10.13995/j.cnki.11-1802/ts.038975

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