In this article, the microbial community structure in pit mud of
different quality was analyzed by high-throughput sequencing and pit mud’s
enzyme activity was also detected. and then to analyze the correlations among
them. The results showed that:The activity of
protease, acid phosphatase, catalase increased with quality improvement of the
pit mud; But the activity of urease decreased with the quality improvement of
pit mud, Dehydrogenase showed the highest activity in the superior pit mud, no
significant difference between the secondary and tertiary pit mud. A total of 2050 OTU were obtained in three grades of pit mud,The number
of OTU in primary pit mud accounts for 50.6% of the total OUT,More than the sum
of the secondary and tertiary OTU number in pit mud;Microbodies of all pit mud are mainly distributed in
9 phylums and 39 genuses. Firmicutes play a leading role in the pit mud , The proportion
of Sporobacter, Coriobacterineae,etc
in pit mud has a positive correlation with the quality of pit mud.Correlation
analysis between enzyme activity and microbial community showed that protease,
acid phospha- tase, catalase and dehydrogenase represented significant with
microbial community in pit mud. The enzyme activity
and microbial community structure in pit mud of different quality all showed
certain regularity, and the enzyme activity of pit mud can be used as an
indicator to reflect the quality of pit mud to make further analysis.
WU Shu-kun et al
. Relation of microbial composition and enzyme activity index with different grades of Luzhou fl avored clay[J]. Food and Fermentation Industries, 2018
, 44(1)
: 19
.
DOI: 10.13995/j.cnki.11-1802/ts.015418