Isolation of dominant lactic acid bacteria from Xinjiang dried meat and analysis of its enzyme-producing characteristics

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  • 1.(Institute of agro-products processing science and technology Xinjiang academy of agricultural and reclamation science, Shihezi, 832000,China) 2. (Key Laboratory of Agro-Products Processing,XinJiangAcademyof Agricultural And Reclamation Science, Shihezi, 832000,China)

Online published: 2018-02-02

Abstract

The dominant lactic acid bacteria were screened in Xinjiang Air-dried meat,and the enzyme production characteristics were alsostydied. Using culture methods, lactic acid bacteria were isolated and screened from Xingjiang Air-dried meat. The isolates were identified by standard physiological and biochemical tests and analysis of 16SrRNA gene sequencing technique. Studied the enzyme production capacity on the different temperature. The results showed that three isolation strain were Lactobacillus plantarum (tL-4), Lactobacillus coryniformis (tL-7), and Lactobacillus pentosus (aL-16). tL-4 and aL-16 showed relatively higher lactic dehydrogenase activity than tL-7.The values reached 112.86.34 U/mL and 94.62 U/mL, respectively. aL-16 presented relatively high nitrite reductase activity. The values reached 14.72 U/mL, respectively. Lactobacillus coryniformis possessed the lowest nitrite reductase activity (4.63U/mL). Compared with the aL-16, tL-7and tL-4 displayed higher esterase activity. The values reached 13.25 U/mL and 11.45U/mL, respectively. Inter-strain differences were presented in the optimal enzyme-producing temperature among the three strains and the optimum conditions for the same strain to produce various enzymes were different. The optimum temperatures for the isolated LAB strains to produce various enzymes were 30 ℃-40℃ respectively. Beyond this temperature range, the enzyme producing ability became weaker.

Cite this article

LI Yu-hui et al . Isolation of dominant lactic acid bacteria from Xinjiang dried meat and analysis of its enzyme-producing characteristics[J]. Food and Fermentation Industries, 2018 , 44(1) : 80 . DOI: 10.13995/j.cnki.11-1802/ts.015523

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