Hops is an important raw material in beer brewing industries. However, the adulteration of the commercial hops has seriously impact the stability of beer quality. Therefore, it is necessary to develop a robust purity detection method for commercial hops. Based on the known genomic database online and sequencing information of this study, primers were designed to amplify the target SNP markers. The resulting 8 polymorphic SNP loci that could distinguish 7 hops cultivars were screened and confirmed. Using Kompetitive allele specific PCR (Kompetitive Allele Specific PCR, KASP) technology based on SNP(Single Nucleotide Polymorphisms), 2 corporate samples and 8 pre-mixed samples were well characterized. The results showed that adulterated hops lower than 5% could be identified using this method. This method can be used to monitor the quality of raw materials in beer and other relative food industries in the future.