Food and Fermentation Industries

High density fermentation of recombinant Kluyveromyces lactis producing antimicrobial peptide PSI andpurifcation of its products

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  • 1 (School of Chemical and Biological Engineering; Lanzhou Jiaotong University; Lanzhou 730070; China) 2 (Key Laboratory of Extreme Environmental Microbial Resources and Engineering; Northwest Institute of Eco-Environment and Resource; Chinese Academy of Sciences; Lanzhou 730000; China)

Online published: 2018-05-25

Abstract

high density fermentation technology for the production of antimicrobial peptide PSI in a 100 L fermentor using a recombinantKluyveromyces lactisstrain was reported in this paper. The effects of mediums; cell density; as well as the inducer lactose and its fed-batch models; on the production of PSI were investigated. Results showed that 5 kg inducer lactose should be added with feeding velocity of 1 L/h when the cell density reach 190 g/L during the fermentation using BSM medium. After 25 h of induction culture; the cell wet weight; the protein yield; and the titer of PSI reached 280 g/L; 27.8 mg/L; and 2.36×109U/L; respectively. The separation and purification of the PSI in the fermentation broth were also investigated. The purification efficiency of anion exchange chromatography; ultrafiltration; precipitation of ammonium sulphate or acetone were 8.83×109; 6.35×109; 4.56×109; and 3.28×109U/L; respectively; which indicated that the most efficient technique for purification of PSI was anion exchange chromatography.

Cite this article

ZHAI Li-xiang1; LI Guo-li1; LENG Yan1;LI Shi-weng1;2; CHEN Xi-ming2; CHEN Tuo2; LIU Guang-xiu2 . High density fermentation of recombinant Kluyveromyces lactis producing antimicrobial peptide PSI andpurifcation of its products[J]. Food and Fermentation Industries, 2018 , 44(4) : 13 -21 . DOI: 10.13995/j.cnki.11-1802/ts.015842

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