Food and Fermentation Industries

Simultaneous determination of 6 kinds of phenolic acids in jujube by GC-MS

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  • (College of Food Engineering and Nutritional Science; Shaanxi Normal University; Xi′an 710119; China)

Online published: 2018-05-25

Abstract

GC-MS was used to simultaneously determine six kinds of phenolic acids in jujube including salicylic acid; 4-hydroxybenzoic acid; vanillic acid; 2;5-dihydroxybenzoic acid; protocatechuic acid and p-coumaric acid. Meanwhile; the column; temperature programmed and derivatized condition were optimized. Finally; the Rtx-5 capillary column (0.25 mm×30 m; 0.25 μm) was chosen. The temperature program was initially at 100 ℃; then raised to 200 ℃ at a rate of 10 ℃/min and then increased at 5 ℃/min up to 250 ℃ and held for 5 min. The total running time was 25 min. The samples were derivatized by BSTFA(containing 1% TMCS)at 35 ℃ for 30min. Six kinds of phenolic acids were completely separated under this optimum condition. The phenolic acid content and the peak area showed a good linear relationship(r2≥ 92.6). The detection limits were 1-6 μg/L; the recoveries were 92.6%-104.3%; and the relative standard deviation were less than 5%. This method has been applied for the simultaneous determination of six kinds of phenolic acids in jujube. The study provides a reference to determine these six kinds of phenolic acids in other products as well.

Cite this article

HAN Xue; ZHANG Fu-xin; SHAO Yu-yu; PENG Hai-xia; WANG Bi-ni* . Simultaneous determination of 6 kinds of phenolic acids in jujube by GC-MS[J]. Food and Fermentation Industries, 2018 , 44(4) : 220 -225 . DOI: 10.13995/j.cnki.11-1802/ts.015267

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