In order to understand the effects of different regions on the processing characteristics of blueberry fruits; four varieties of blueberries from 6 different regions of Majiang County in Guizhou province were investigated and analyzed by using the principal component analysis and cluster analysis. The results showed that the indexes of blueberry varied at different degrees in different varieties and regions. According to the principal component analysis; the 14 indexes reduced to 6 indicators; which reflects 87.3% of the original variables. According to the comprehensive score of 6 principal components weights; the scores of gardenblue and powderblue grow in Langjiang and Dashenglin are higher than in the other regions; the scores of Baldwin and Bright well grow in Longben and Biebo are higher than other regions; the scores of Baldwin and Bright well grow in Langjiang and Dashenglin is relatively low. According to the cluster analysis; 16 blueberry samples were clustered into 4 classes; Baldwing in Longben and Bright well in different regions were clustered into one class; and the rest of blueberry samples were clustered into another one. In summary; the effects of regions on the quality of different varieties were different; the adaptability of different varieties was different. Compared with varieties; the cultivated place has less impact.