In order to obtain the numb-taste components of Zanthoxylum in an economic and efficient way, molecular imprinting polymers (MIPs) were prepared through bulk polymerization using dummy template(LM2) as molecule template. The adsorption dynamic curve of MIPs displayed that adsorption of numb-taste components of Zanthoxylum on MIPs could reach equilibrium within 10h, and the recognition factor (α) was 2.53. The adsorption isothermal curves of MIPs and NIPs showed that adsorption quantity of numb-taste components of Zanthoxylum in the cavity formed by MIPs was higher than that of NIPs. There are two kinds of the binding sites between imprinted polymer and numb-taste components. The result shows that using the dummy template(LM2) as the template molecule, SMIP has a better imprinting effect.
LI Yao
,
HE Ye-zi
,
CHEN Xiao-long
,
KAN Jian-quan
. Preparation and binding characteristics of dummy molecularly imprinted polymers[J]. Food and Fermentation Industries, 2018
, 44(9)
: 78
-83
.
DOI: 10.13995/j.cnki.11-1802/ts.016391
[1] 张敬文, 赵镭, 史波林, 等. 花椒果皮中化学成分的研究[J]. 华西药学杂志, 2016, 31(2): 109-112.
[2] YANG X. Aroma constituents and alkylamides of red and green huajiao (Zanthoxylum bungeanum and Zanthoxylum schinifolium)[J]. Journal of Agricultural and Food Chemistry, 2008, 56(5): 1 689-1 696.
[3] TEZUKA Y, IRIKAWA S, KANEKO T, et al. Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bungeanum[J]. Journal of Ethnopharmacology, 2001, 77(2/3): 209-217.
[4] WANG Shi, XIE Jian-chun, YANG Wei, et al. Preparative separation and purification of alkylamides from Zanthoxylum bungeanum maxim by high-speed counter-current chromatography[J]. Journal of Liquid Chromatography & Related Technologies, 2011, 34(20): 2 640-2 652.
[5] 许志刚, 刘智敏, 杨保民, 等. 替代模板分子印迹技术在样品前处理中的应用[J]. 化学进展,2012, 24(8): 1 592-1 598.
[6] 张红武, 郭建文, 李康, 等. 以局部结构类似物为类模板的分子印迹整体柱在线检测三甲氧苄啶[J]. 分析化学,2012, 40(5): 699-704.
[7] 高文惠, 高林, 刘博, 等. 制备戊唑醇分子印迹聚合物前功能单体的选择[J]. 中国食品学报, 2015,15(6): 173-177.
[8] 宋兴良, 王爱香, 刘晓泓, 等. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 2016, 35(3): 451-459.
[9] 权英, 孙静, 沈阳, 等. 丙烯酰胺分子印迹聚合物的制备及性能研究[J]. 食品与发酵工业, 2010(12): 177-180.
[10] 罗凯,胡江,黄秀芳, 等.花椒麻味物质的提取分离及其组分分析[J]. 食品科学, 2013, 34(24): 88-91.
[11] MENOZZISMARRITO C, RIERA C E, MUNARI C, et al. Synthesis and evaluation of new alkylamides derived from α-hydroxysanshool, the pungent molecule in szechuan pepper[J]. Journal of Agricultural & Food Chemistry, 2009, 57(5): 1 982-1 989.
[12] 杜文倩, 史波林, 欧克勤, 等. 基于麻味物质构成特征的红花椒高效液相色谱指纹图谱建立研究[J]. 食品安全质量检测学报, 2016, 7(3): 1 138-1 144.
[13] MA X, JI W, CHEN L. Molecularly imprinted polymers of capsaicin[J]. Journal of Separation Science, 2015, 38(1): 100-107.
[14] SUN J, PENG N, AO W X, et al. Preparation and Binding Characteristics of Molecularly Imprinted Polymers for Capsaicin[J]. Advanced Materials Research, 2012, 2012, 430-432(4): 582-585.
[15] SHAHANA S. KHURSHID, CHRISTINE E, et al. Optimization of molecularly imprinted polymers of serotonin for biomaterial applications[J]. Journal of Biomaterials Science Polymer Edition, 2011, 22(1-3):343-362.
[16] CHEN Z, YE L. Controlling size and uniformity of molecularly imprinted nanoparticles using auxiliary template. Journal of Molecular Recognition[J]. 2012, 25(6): 370-376.
[17] 王尊臣, 于宁海, 单伟光, 等, 辣椒素分子印迹聚合物的制备及其在固相萃取中的应用[J].食品科学, 2013,34(12): 45-50.
[18] LI M, ZHU Y, LI L, et al. Molecularly imprinted polymers on a silica surface for the adsorption of tobacco-specific nitrosamines in mainstream cigarette smoke[J]. Journal of Separation Science, 2015, 38(14): 2 551-2 557.
[19] MA L, TANG L, LI R S, et al. Water-compatible molecularly imprinted polymers prepared using metal-organic gel as porogen[J]. RSC Adv, 2015, 5(103): 84 601-84 609.
[20] 李耀, 陈晓龙, 阚建全, 等. 花椒麻味物质分子印迹聚合物的制备及吸附特性[J]. 食品科学, 2017, 38 (19): 35-41.
[21] 宋兴良, 王爱香, 刘晓泓, 等. 虚拟模板分子印迹微球的制备及其对水中微囊藻毒素的吸附性能[J]. 环境化学, 2016, 35(3): 451-459.
[22] GAO D, ZHANG Z, WU M, et al. A surface functional monomer-directing strategy for highly dense imprinting of TNT at surface of silica nanoparticles[J]. Journal of the American Chemical Society, 2007, 129(25): 7 859-7 866.
[23] MA Xiu-li, JI Wen-hua, CHEN Ling-xiao, et al. Molecularly imprinted polymers with synthetic dummy templates for the preparation of capsaicin and dihydrocapsaicin from chili peppers[J]. Journal of Separation Science,2015,28(1): 100-106.
[24] XU W Z, ZHOU W, XU P P, et al. A molecularly imprinted polymer based on TiO2 as a sacrificial support for selective recognition of dibenzothiophene[J]. Chemical Engineering Journal, 2011, 172(1): 191-198.
[25] 孙妍, 王兵. 单一结合位点分子印迹聚合物的合成及性能[J]. 高分子材料科学与工程, 2012(5): 8-11.
