The four different washing methods of fresh-cut lotus root including ozone, ultrasound, ozone combined with ultrasound and distilled water were studied.After washing, the lotus roots were stored under 4 ℃.Color values.Browning degree (BD), polyphenol oxidase (PPO) activity, total number of colonies other quality indicators were examined every two days during the storage period.Twelve traits of the effect of O3 and ultrasound on the quality of fresh-cutting lotus root were analyzed by principal component analysis (PCA) method.An assessment model was established to screen the best treatment way.Two principal components were screened out through principal component analysis which accounted for 85.858% of the total variance.Comprehensive principal component analysis showed that the best quality score was cleaning by ozone combined with ultrasound, the group maintained the color of lotus root，inhibit browning, reduce the PPO activity, maintain a high soluble solid.Therefore, the combination of ultrasound and O3 treatment was efficient in the preservation of lotus roots.