A solid-phase microextraction coupled with gas chromatography-tandem mass spectrometry method was developed for the determination of 35 polychlorinated biphenyls in aquatic vegetables.Samples were extracted with 100 μm polydimethylsiloxane at 60 ℃ for 30 min, and stirring at 250 r/min.After desorbed 6 min at 280 ℃ in gas chromatograph, sample extracts were injected and analyzed by GC-MS/MS at time selective reaction monitoring mode, and quantified by external standard method.The results showed that 35 target analysts displayed good linear relationships over concentration range of 10-500 μg/L, and correlation coefficients above 0.999.The limits of detection were 0.55-17.22 ng/kg.The average recoveries of 35 compounds at two spiked levels of 0.01 mg/kg ranged from 72.3% to 117.3%, with relative standard deviations of 1.2% to 11.2%.The advantages of simplicity, rapidness, sensitiveness and accuracy of the method was suitable for rapid detection of PCBs residues in aquatic vegetables.