A macroporous and weak acid cation exchange resin, Na+-form Amberlite IRC-50, was employed to separate and purify ε-poly-L-lysine (ε-PL) from fermentation broth. Based on the optimization of uploading, wishing and elution parameters, the optimal dynamic ion exchange conditions were developed as following: the sample was uploaded at pH 7.0 with loading rate at 5 BV/h; deionized water was used for washing resin at 2 BV/h; 0.25 mol/L NaOH was used as eluent with elution rate at 2 BV/h. Under the above conditions, the working exchange capacity of the resin was reached by 305.12 mg/g, and the desorption rate was attained to more than 95%. Finally, the purity of ε-PL was enhanced from the initial 24.29% to 75.26%, and the ε-PL recovery rate was also reached by 96.40%.
HE Hong-gang et al
. Optimization of conditions for separation and extraction of ε-poly-L-lysine fromfermentation broth by Na + form Amberlite IRC-50 resin[J]. Food and Fermentation Industries, 2018
, 44(7)
: 148
-153
.
DOI: 10.13995/j.cnki.11-1802/ts.013723