Glucose oxidase is an aerobic dehydrogenase that specifically catalyzes the oxidation of β-D-glucose to produce gluconic acid and hydrogen peroxide. It has great application value in food, medicine, chemical industry, textile, biotechnology and other fields. Microbes are the main source of glucose oxidase, which have the advantages of fast growth and reproduction, high efficiency of enzyme production, and simple purification proces. This paper reviews the microbial sources of glucose oxidase, the genetic engineering transformation of enzyme producing strains and the application of glucose oxidase. It provides a theoretical reference for the development of glucose oxidase-producing microorganism and the application of genetic engineering technology in strain selection.