Mutagenesis effect of space mutation on Streptococcus thermophilus and its fermentation performance evaluation

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  • 1(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070, China) 2(College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100083, China) 3(Hebei Engineering Research Center for Animal Product,Sanhe 065200, China) 4(Mengniu Hi-tech Dairy(Beijing)Co., Ltd., Beijing 101107, China) 5(School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China)

Online published: 2018-09-13

Abstract

In this experiment, 10 strains of Streptococcus thermophilus strain from Shenzhou-11 return spacecraft was isolated and purified. The growth curve, titration acidity, viscosity and extracellular polysaccharide content of wild strain G2 and test strains were measured, and scanning electron microscopy (SEM) and transmission electron microscopy (TEM) tests were performed. The results showed that OD600 value of two strains B2-1 and B2-4 (2.073±0.15, 2.06±0.11), titration acidity (106±0.23) °T, (98.5±0.25)°T, viscosity (3.27±0.04) Pa·s, (2.94±0.01)Pa·s, and Extracellular polysaccharides (409.35±1.38) mg/L, (398.80±5.38) mg/L were all higher or significantly higher than those of wild strain G2 with OD600 of (2.051±0.11 ), Titration acidity of (92±0.16) °T, viscosity of (2.66±0.07 )Pa·s and extracellular polysaccharide content of (385.11±3.074) mg/L. Finally, two strains of Streptococcus thermophilus with excellent characters of B2-1 and B2-4 were obtained. Therefore, space mutation breeding may change the Streptococcus thermophilus fermentation performance.

Cite this article

WANG Ying et al . Mutagenesis effect of space mutation on Streptococcus thermophilus and its fermentation performance evaluation[J]. Food and Fermentation Industries, 2018 , 44(8) : 65 -70 . DOI: 10.13995/j.cnki.11-1802/ts.017045

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