Rice is an important adjunct during beer brewing. Adding rice changed the concentration and physicochemical properties of beer protein. In this study, the effect of adding rice on the hydrophobicity and foaming stability of protein was mainly investigated. With the increase of the amount of rice addition from 10% to 30%, the hydrophobic index of beer protein gradually decreased from 118.86 to 103.87, the foaming stability of protein increased from 62.14% to 68.25%. The beer protein from rice was analyzed by SDS-PAGE. It was found that rice beer contains less protein Z and a certain amount of RAG2 protein, which had an effect on beer foam stability.
CAO Rong-kun
,
LI Jia-tai
,
WANG Jin-jing
,
ZHENG Fei-yun
,
NIU Cheng-tuo
,
LIU Chun-feng
,
LI Yong-xian
,
LI Qi
. Hydrophobicity and foaming stability analysis of rice beer protein[J]. Food and Fermentation Industries, 2018
, 44(9)
: 66
-70
.
DOI: 10.13995/j.cnki.11-1802/ts.017253
[1] 顾国贤. 酿造工艺学[M]. 北京: 中国轻工业出版社, 1996:1-4.
[2] 周林生. 不同品种大米在啤酒酿造中的应用研究[D]. 无锡: 江南大学, 2008.
[3] SHOKRIBOUSJEIN Z, DECKERS S M, GEBRUERS K, et al. Hydrophobins, beer foaming and gushing[J]. Cerevisia, 2011, 35(4): 85-101.
[4] 陈熙, 王金晶, 杨晗,等. 啤酒组分与泡沫稳定性的相关性研究[J]. 食品与发酵工业, 2014, 40(7):29-33.
[5] 田金凤, 王金晶, 郑飞云,等. 啤酒主要组分在泡沫中的富集及其对泡持性的影响[J]. 食品与发酵工业, 2017, 43(9):22-27.
[6] 韩宇鹏. 麦芽蛋白质Z稳定啤酒泡沫机制的研究[D]:江南大学, 2017.
[7] 韩宇鹏, 王金晶, 田金凤,等. 啤酒蛋白及其理化特性研究进展[J]. 食品与发酵工业, 2016, 42(9):270-276.
[8] 穆利霞, 崔春, 赵谋明,等. 阿拉伯胶-大豆分离蛋白接枝工艺优化及产物理化特性的研究[J]. 食品工业科技,2009(8):174-177.
[9] SUN Y, SHIGERU HAYAKAWA A, IZUMORI K. Modification of ovalbumin with a rare ketohexose through the maillard reaction: effect on protein structure and gel properties[J]. J Agric Food Chem, 2004, 52(5): 1 293-1 299.
[10] 余晶梅. 荧光探针法和疏水相互作用层析法分析蛋白表面疏水性[D]. 杭州:浙江大学, 2014.
[11] MIYAZAWA S, JERNIGAN R L. Estimation of effective interresidue contact energies from protein crystal structures: quasi-chemical approximation[J]. Macromolecules, 1985, 18(3):534-552.
[12] HUANG X, TU Z, WANG H, et al. Increase of ovalbumin glycation by the maillard reaction after disruption of the disulfide bridge evaluated by liquid chromatography and high resolution mass spectrometry[J]. Journal of Agricultural and Food Chemistry, 2013, 61(9): 2 253-2 262.
[13] HORAX R,HETTIARACHCHY N,OVER K,et a1.Extraction fractionation and characterization of bitter melon seed proteins[J].Journal of Agricultural and Food Chemistry,2010,58(3):1 892-1 897
[14] 陈卓, 侯俊杰, 杨晓泉. 米黑毛酶提高大豆分离蛋白泡沫稳定性的研究[J]. 中国粮油学报, 2014, 29(3):52-56.
[15] JAIN A, PRAKASH M, RADHA C. Extraction and evaluation of functional properties of groundnut protein concentrate[J]. Journal of Food Science and Technology, 2015, 52(10): 6 655-6 662.
[16] MORO A, GATTI C, DELORENZI N. Hydrophobicity of whey protein concentrates measured by fluorescence quenching and its relation with surface functional properties[J]. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4 784-4 789.
[17] DIDIER M. Soluble proteins of beer[M]. Beer in Health and Disease Prevention, 2009: 265-271.
[18] 单成俊 , 周剑忠 . 大米蛋白研究进展及其应用[J]. 粮食与饲料工业 , 2009(7):30-33.
[19] 陶林, 王德良, 曹健,等. 啤酒中疏水性蛋白质提取鉴定及其对于泡沫性能的影响的研究[J]. 中国酿造, 2011, 30(5):116-120.