The non-volatile component of Baijiu (Chinese liquor) has an important effect on overall flavor of the liquor. This study applied taste dilution analysis (TDA) to analyze the taste compounds in non-volatile component of Chinese Moutai-flavor type liquor. The experiment used human taste as a guide, combining with solid phase extraction and high performance liquid chromatography separation, to screen three taste-active fractions in non-volatile component of the liquor sample. These fractions were further analyzed by means of high resolution mass spectrometry and nuclear magnetic resonance. For the first time, bitter-tasting compounds 9,10,13-trihydroxyoctadec-11(E)-enoic acid (1) and 9,12,13-trihydroxyoctadec-10(E)-enoic acid (2) were identified in Chinese liquor. Based on ultra-high performance liquid chromatography-tandem mass spectrometry, a precise quantitative method for detection of the two trihydroxyoctadec acids in Chinese liquor was developed. The quantitative result revealed that compounds 1 and 2 were distributed in Chinese Moutai, strong and light-flavor type liquor samples (n=6), with range of 1.8-265.3 and 2.2-111.3 μg/L, respectively. Moreover, their concentrations in Moutai-flavor type liquor samples were significantly higher than those of other flavor types (p<0.01).
ZHAO Teng-fei
,
CHEN Shuang
,
LI Hua-zhong
,
XU Yan
. Isolation and identification of bitter-tasting trihydroxyoctadecenoic acids in Baijiu(Chinese liquor)[J]. Food and Fermentation Industries, 2018
, 44(9)
: 225
-231
.
DOI: 10.13995/j.cnki.11-1802/ts.017511
[1] JIN Guang-yuan, ZHU Yang, XU Yan. Mystery behind Chinese liquor fermentation[J]. Trends in Food Science & Technology, 2017, 63: 18-28.
[2] ZHANG Rong, WU Qun, XU Yan, et al. Isolation, identification, and quantification of lichenysin, a novel nonvolatile compound in Chinese distilled spirits[J]. Journal of Food Science, 2014, 79(10): C1 907-C1 915.
[3] 郑伟. 白酒非挥发性组分研究[D]. 南京: 南京理工大学, 2007.
[4] 徐岩. 基于风味导向技术的中国白酒微生物及其代谢调控研究[J]. 酿酒科技, 2015(2): 1-11;16.
[5] FAN Hai-yan, FAN Wen-lai, XU Yan. Characterization of key odorants in Chinese chixiang aroma-type liquor by gas chromatography-olfactometry, quantitative measurements, aroma recombination, and omission studies[J]. Journal of Agricultural and Food Chemistry, 2015, 63(14): 3 660-3 668.
[6] WANG Xiao-qin, FAN Wen-lai, XU Yan. Comparison on aroma compounds in Chinese soy sauce and strong aroma type liquors by gas chromatography-olfactometry, chemical quantitative and odor activity values analysis[J]. European Food Research and Technology, 2014, 239(5): 813-825.
[7] SHA Sha, CHEN Shuang, XU Yan, et al. Characterization of the typical potent odorants in Chinese roasted sesame-like flavor type liquor by headspace solid phase microextraction-aroma extract dilution analysis, with special emphasis on sulfur-containing odorants[J]. Journal of Agricultural and Food Chemistry, 2017, 65(1): 123-131.
[8] 杨会, 范文来, 徐岩. 基于BSTFA衍生化法白酒不挥发有机酸研究[J]. 食品与发酵工业, 2017, 43(5): 192-197.
[9] MOODY W G. Beef flavor-a review[J]. Food Technology, 1983, 37: 227-238.
[10] FARMER L J. The role of nutrients in meat flavour formation[J]. Proceedings of the Nutrition Society, 1994, 53(2): 327-333.
[11] FRANK O, OTTINGER H, HOFMANN T. Characterization of an intense bitter-tasting 1H,4H-quinolizinium-7-olate by application of the taste dilution analysis, a novel bioassay for the screening and identification of taste-active compounds in foods[J]. Journal of Agricultural and Food Chemistry, 2001, 49(1): 231-238.
[12] DRESEL M, DUNKEL A, HOFMANN T. Sensomics analysis of key bitter compounds in the hard resin of hops (Humulus lupulus L.) and their contribution to the bitter profile of pilsner-type beer[J]. Journal of Agricultural and Food Chemistry, 2015, 63(13): 3 402-3 418.
[13] HASHIZUME K, ITO T, SHIMOHASHI M, et al. Taste-guided fractionation and instrumental analysis of hydrophobic compounds in sake[J]. Bioscience Biotechnology and Biochemistry, 2012, 76(7): 1 291-1 295.
[14] HUFNAGEL J C, HOFMANN T. Orosensory-directed identification of astringent mouthfeel and bitter-tasting compounds in red wine[J]. Journal of Agricultural and Food Chemistry, 2008, 56(4): 1 376-1 386.
[15] 张跃廷. 白酒中的 “苦味” 浅议[J]. 酿酒, 2011, 38(1): 61-62.
[16] 陈育新, 韩珍, 郭庆东. 中国白酒中呈香呈味物质研究进展[J]. 食品研究与开发, 2015, 36(2): 140-142.
[17] MARTIN-ARJOL I, BASSAS-GALI M, BERMUDO E, et al. Identification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2[J]. Chemistry and Physics of Lipids, 2010, 163(4-5): 341-346.
[18] BAUR C G, WIESER W, JUGEL H. Enzymatic oxydation of linoleic acid: formation of bittertasting fatty acids[J]. Z Lebensm Unters Forsch, 1977, 164: 171-176.
[19] BIN Q, PETERSON D G. Identification of bitter compounds in whole wheat bread crumb[J]. Food Chemistry, 2016, 203: 8-15.
[20] HAMBERG M. Trihydroxyoctadecenoic acids in beer-qualitative and quantitative-analysis[J]. Journal of Agricultural and Food Chemistry, 1991, 39(9): 1 568-1 572.
[21] PUSSA T, RAUDSEPP P, TOOMIK P, et al. A study of oxidation products of free polyunsaturated fatty acids in mechanically deboned meat[J]. Journal of Food Composition and Analysis, 2009, 22(4): 307-314.
[22] 袁蕊, 敖宗华, 丁海龙, 等. 高粱中脂肪酸和低分子有机酸气相色谱测定[J]. 酿酒, 2011, 38(4): 42-43.
