The regular patterns of KMOS utilization by three different bacteria (Bifidobacterium adolescentis, Bacteroides uniformi, Lactobacillus johnsonii) was investigated under anaerobic and static conditions. The results showed that Bacteroides uniformis could quickly and completely consumed DP 2～3, whereas Lactobacillus johnsonii could utilize these components slowly but with a significant lag phenomenon. Different with the former two, Bifidobacterium adolescentis could only consume DP 2 completely and DP 3～4 partly. All of the 3 bacteria could grow in the culture with KMOS and produce organic acid (mainly lactic acid and succinic acid) and SCFA (mainly acetic acid, butanoic acid, butylcarboxylic acid and hexylic acid), but had significant discrepancy in the rate and content. These results could provide reference for developing prebiotics product of KMOS.